对《中国药典》2010年版丁香药材中丁香酚含量测定方法的探讨  

Discussion about Assay Method of Eugenol in Flos Caryophylli in Chinese Pharmacopoeia(Edition 2010)

作　　者：黎迎[1] 陆洋[1] 杜守颖[1] 王英姿[1] 萧伟[2] 王玥[1] 徐攀[1] 刘月环[1] 赵静宜[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《中药新药与临床药理》2012年第5期579-582,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：中药制药过程新技术国家重点实验室开放基金课题(SKL2010M0302);北京中医药大学自主选题项目(2011JYB22XS-063);北京中医药大学复方中药制剂研究创新团队发展计划资助项目(2011-CXTD-13)

摘　　要：目的改进丁香药材中丁香酚的含量测定方法。方法HPLC法测定丁香酚的含量，流动相为甲醇-1％醋酸水（55：45），检测波长为280nm，并与《中国药典》2010年版一部的测定方法进行比较。结果新建方法测定丁香中丁香酚的含量高于《中国药典》方法，新方法灵敏、准确、重现性好。丁香酚在0．1605—9．6504ug范围内线性关系良好，r=0．9999，平均加样回收率为100．27％，RSD=0．82％。结论HPLC测定法可用于丁香药材的质量控制，为改进丁香的含量测定方法提供依据。Objective To improve the method of determination of eugenol in Flos Caryophylli. Methods The content of eugenol was determined by HPLC at 280 nm detection wavelength with methanol-1% glacial acetic acid （55 ： 45） as mobile phase. The modified assay method of eugenol in Flos Caryophylli was compared with the pharmacopoeia method. Results The modified method for determination of eugenol was superior to the pharmacopoeia method. This method was sensitive, accurate and reproducible. Eugenol in the range of 0.1605-9.6504 ug was linear, r=0.9999, average recovery was 100.72 %, and RSD=0.82 %. Conclusion The modified method can be used for the quality control of Flos Caryophylli, and the results will supply evidence for the assay of Flos Caryophylli.

关 键 词：丁香 丁香酚 含量测定 甲醇回流 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]