枣树幼叶总RNA提取方法的比较研究  

Comparison of Four Methods for Extracting of Total RNA From Young Jujube Leaves

作　　者：陈国梁[1] 陈宗礼[1] 孙萍[1] 杨清湖[1] 刘咪咪[1] 

机构地区：[1]延安大学生命科学学院,陕西省红枣重点实验室,陕西省区域生物资源保育与利用工程技术研究中心,陕西延安716000

出　　处：《北方园艺》2012年第19期131-133,共3页Northern Horticulture

基　　金：陕西省自然科学基金资助项目(S2009JC970);陕西省教育厅自然基金资助项目(11JK0617);延安市科学技术研究发展计划资助项目(2010kn-03);延安大学专项科研基金资助项目(YD2010-19);延安大学大学生科技创新训练计划资助项目(D2011-43)

摘　　要：以枣树幼嫩叶片为试材,分别采用DEPC水法、SDS抽提法、CTAB-LiCl和异硫氰酸胍法4种方法进行总RNA的提取试验,并对其进行了紫外、变性琼脂糖凝胶电泳及RT-PCR检测。结果表明:4种提取方法中DEPC水法提取的总RNA质量最好,SDS抽提法和CTAB-LiCl法次之,异硫氰酸胍法效果最差。Taking young jujube leaves as material,RNA in jujube leaves was extracted by the DEPC water method,SDS method,CTAB-LiC1 method and isothiocyanate guanidine method. The quality of the total RNA was tested by nuclear acid/protein analyzer, denaturation agarose gel electrophoresis and RT-PCR. The results showed that total RNA extracted by DEPC water method had the best quality and reliability, followed by SDS extraction method and CTAB-LiC1 method, the isothiocyanate guanidine method was the worst.

关 键 词：枣树叶片 总RNA 提取方法 电泳 

分 类 号：Q75[生物学—分子生物学]