石榴叶片蛋白提取方法研究  

Study on Protein Extraction Methods of Pomegranate Leaves

作　　者：田忠景[1] 康美玲[1] 王秀文[1] 

机构地区：[1]枣庄学院生命科学学院,山东枣庄277160

出　　处：《北方园艺》2012年第19期137-139,共3页Northern Horticulture

摘　　要：以"大红袍"石榴叶片为试材,研究比较了三氯乙酸(TCA)/丙酮沉淀法、Tris-HCl提取法和酚-甲醇/醋酸铵沉淀法对石榴叶片总蛋白质的提取效果间的差异。结果表明:TCA/丙酮沉淀法所得电泳图谱背景不清晰;酚-甲醇/醋酸铵沉淀法步骤繁琐,蛋白质信息量少;Tris-HCl提取法为最适方法,所得图谱背景清晰,蛋白质信息量最大。Taking the leaves of Punicagranatum Linn ‘Dahongpao’as material,three protocols for total protein extraction,trichloroacetic acid/acetone precipitation（TCA）,phe-nol extraction methanol/ammonium acetate precipitation and Tris-HCl extraction were compared.The results showed that TCA/Acetone extration was unclear background;the Tris-HCl extraction was the most appropriate for pomegranate leaf proteome analysis on account of the highest resolution and more informative.

关 键 词：石榴 蛋白质组 垂直板电泳 考马斯亮蓝染色 

分 类 号：Q51[生物学—生物化学]