Bcl-2在小鼠肾脏发生发育中的表达  

Expression of Bcl-2 during the development of mouse kidney

作　　者：宋小峰[1,2] 陈雪[1] 任昊[1] 翟效月[1] 

机构地区：[1]中国医科大学组织学与胚胎学教研室,沈阳110001 [2]辽宁医学院组织学与胚胎学教研室,辽宁锦州121001

出　　处：《解剖学报》2012年第5期658-661,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30871313;31171400);辽宁省自然科学基金资助项目(201202146)

摘　　要：目的探讨小鼠肾脏发育过程中抗凋亡蛋白Bcl-2的表达。方法选取胚龄16(E16d)、18d和出生后1d(P1d)、3、5、7d、14和21d的昆明小白鼠共54只,应用免疫组织化学技术并结合体视学分析方法,观察小鼠肾脏不同发育时期树脂切片上Bcl-2的表达。结果在小鼠肾脏发育过程中,Bcl-2的阳性表达主要出现在近端小管,从E18 d到P1 d,Bcl-2阳性表达肾小管面数密度的增长速度最快,在P7 d之后,Bcl-2阳性表达肾小管面数密度的增长速度的变化不大。结论在肾的早期发育过程中,Bcl-2主要影响了近端小管的存活或死亡。Objective To explore the expression of Bcl-2 during mouse kidney development. Methods Fifty- five Kunming mice from embryonic day 16 （E16d） , 18 and postnatal day 1 （P1 d） , 3,5,7, 14, 21 were chosen to be used to investigate the expression of Bel-2 on epoxy sections with immunohistochemistry technique and stereological methods. Results During mouse kidney development, the positive staining of Bcl-2 was mainly observed in proximal tubules. The increase with numerical area density（NA） of Bcl-2 was the fastest from E18d to P1 d, while not much changed after P7 d. Conclusion During the pristine kidney development, the expression of Bcl-2 influences survival or death of proximal tubule.

关 键 词：BCL-2 近端小管 发育 树脂切片 免疫组织化学 免疫荧光 小鼠 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]