非去极化停搏液对缺血再灌注心肌细胞L-型钙通道的影响  

Effect of NDP solution on L-type calcium channel in ischemia-reperfusion neonatal rat myocytes

作　　者：吴蓓[1] 黑飞龙[1] 孙亮[1] 陈蒙蒙[1] 于坤[1] 龙村[1] 

机构地区：[1]北京协和医学院中国医学科学院阜外心血管病医院体外循环科,北京100037

出　　处：《中国体外循环杂志》2012年第3期162-166,共5页Chinese Journal of Extracorporeal Circulation

基　　金：国家自然科学基金(30972866);北京市自然科学基金(7102131)

摘　　要：目的探讨非去极化停搏液(non-depolarizing solution,NDP)对缺血再灌注心肌细胞L-型钙通道(ICa-L)的影响。方法实验分为对照(Con)组、缺血再灌注(I/R)组和非去极化停搏液(NDP)组。选择培养18～48 h的SD乳鼠心肌细胞用于实验。Con组细胞不经过缺血再灌注处理;I/R组细胞经模拟缺血缺氧3 h、再灌注1 h处理;NDP组在模拟I/R过程中,加NDP液于细胞培养基中进行干预。用全细胞膜片钳技术检测ICa-L的电流强度和门控特性。结果与对照组相比,I/R组ICa-L的峰值电流密度明显降低[(-9.0±3.8)pA/pF vs(-15.1±8.8)pA/pF,P<0.05],电流-电压曲线(I-V曲线)上移,翻转电位绝对值减小,稳态激活曲线和失活曲线左移,恢复曲线右移。与I/R组相比,NDP组ICa-L的峰值电流密度升高[(-11.4±6.7)pA/pF vs(-9.0±3.8)pA/pF,P<0.05],I-V曲线下移,翻转电位绝对值增大,稳态激活曲线和失活曲线右移,恢复曲线左移。结论 NDP液可减轻心肌细胞I/R损伤对ICa-L通道的抑制作用和门控特性的改变,有利于保护心肌收缩和舒张功能、减少心律失常的发生。Objective To study the effect of nondepolarizing （NDP） cardiac preservation solution on L- type calcium channel （Ica- L ） in ischemia -reperfusion （I/R） neonatal rat myocytes. Methods Cardiomyocytes were primary cultured for 18 -48 h before using in the experiment. Cells were treated without I/R in the Con group, with I/R in the I/R group and with I/R plus NDP solution in the NDP group. Whole cell patch clamp was used to record the current and gating. ReSults The peak current density in the I/R group decreased significantly than the Con group （ - 9.0 ± 3.8 pA/pF vs - 15.1 ± 8.8 pA/pF, P 〈 0.05 ）. Compared with the Con group, the I - V curve of the I/R group up - shifted, activation curve and inactivation curve left - shifted and recovery curve right - shifted. Compared with the I/R group, the peak current density in the NDP group increased （ - 11.4 ± 6.7 pA/pF vs - 9.0 ± 3.8 pA/pF, P 〈 0.05 ）. The I - V curve of the I/R group down - shifted, activation curve and inactivation curve right - shifted and the recovery curve left - shifted compared with the I/R group. Conclusion NDP solution could alleviate the effect of current inhibition and gating alteration induced by I/R injury in neonatal rat myocytes, which is beneficial to prevent arrhythmias and protect the cardiac contraction and dilatation.

关 键 词：缺血再灌注 L-型钙通道 心肌细胞 全细胞膜片钳技术 

分 类 号：R654.2[医药卫生—外科学]