骨性关节炎患者关节软骨hMLH1启动子区甲基化水平  

hMLH1 gene promoter methylation in joint cartilage in patients with osteoarthritis

作　　者：徐敏[1] 沈奕[1] 张强[1] 陈明辉[1] 余波[1] 彭丹[1] 

机构地区：[1]中南大学湘雅二医院骨科,长沙410011

出　　处：《中南大学学报（医学版）》2012年第9期928-933,共6页Journal of Central South University ：Medical Science

基　　金：湖南省科学技术厅科技计划一般项目(2009FJ3068)~~

摘　　要：目的:通过对骨性关节炎(osteoarthritis,OA)患者关节软骨人类错配修复基因1(human MutL homologue1,hMLH1)启动子区甲基化状态及hMLH1蛋白表达的检测,探讨hMLH1启动子区甲基化在OA发生发展中的作用。方法:采用亚硫酸氢钠法处理基因组DNA,甲基化特异性PCR检测hMLH1基因启动子甲基化情况;免疫组织化学方法检测hMLH1蛋白的表达。结果:OA患者组关节软骨hMLH1启动子区甲基化阳性率明显高于健康人对照组(χ2=30.634,P<0.001);OA患者组关节软骨hMLH1蛋白表达率明显低于健康人对照组(χ2=37.724,P<0.001);OA患者关节软骨hMLH1启动子区启动子甲基化与蛋白表达呈显著负相关(rs=-0.554,P<0.001)。结论:OA患者关节软骨hMLH1启动子区发生了甲基化。其高甲基化状态影响了hMLH1蛋白的表达,可能参与OA疾病的发生发展。Objective： To investigate the role of Human MutL homologue 1 （hMLH1） gene promoter methylation in the occurrence and development of osteoarthritis （OA）. Methods： General DNA was dealt with sodium bisulfite. The methylation of hMLH1 promoter was detected by methylation-specific PCR （MSP）. hMLH1 protein expression in joint cartilage was detected by immunohistochemical method. Results： The positive percent ofhMLH1 promoter methylation in OA patients was higher than that in healthy persons （X2=30.634, P〈0.001）; the positive percent of hMLHI protein in OA patients was significantly lower than that in healthy persons （X2=37.724, P〈0.001）; prmoter methylation and protein expression level ofhMLH1 gene showed negative correlation （rs=0.554, P〈0.001）. Conclusion： hMLH1 promoter is hypermethlated in joint cartilage ceils of OA patients. Hypermethylation may affect the protein expression of hMLH1, which might play a role in theoccurrence and development of OA.

关 键 词：骨性关节炎 DNA甲基化 hMLH1启动子 甲基化特异性PCR 

分 类 号：R684[医药卫生—骨科学]