运用线粒体COⅠ基因分析8种石珊瑚系统发育关系  

Phylogenetic relationship in 8 species of Scleractinian coral based on COⅠ sequences

作　　者：李晓娜[1] 刘丽[1] 刘楚吾[1] 

机构地区：[1]广东海洋大学水产学院南海水产经济动物增养殖广东省普通高校重点实验室,湛江524025

出　　处：《渔业科学进展》2012年第4期83-88,共6页Progress in Fishery Sciences

基　　金：国家海洋公益性行业科研专项(201105012);广东省自然科学基金(S2011010000269);广东省海洋渔业科技推广专项(A200908F01)共同资助

摘　　要：通过COⅠ基因特异扩增测序,对徐闻地区的8种石珊瑚COⅠ基因片段序列进行了比较分析。结果表明,该片段的平均G+C含量为41.60%。其中,第1密码子位点的含量最高(42.26%～44.05%,平均为43.45%);转换和颠换多发生在第3密码子位点,比例高达70.30%和72.70%。采用临位连接(NJ)、最小进化(ME)和最大似然(ML)法对本研究中徐闻的8种及引自GenBank的20种石珊瑚COⅠ基因片段构建了系统发育树。结果显示,分子系统分类与传统分类略有差异,暗示珊瑚表型可塑性可能对传统分类存在影响。Partial sequences of CO I genes from 8 species of Scleractinian in Xuwen area, Guangdong Province, were amplified and sequenced, and the base ratio and variations were ana- lyzed . The result indicated that the average G＋C content of this region accounted for 41.60%, while the eodon position 1 was the highest, in the range of 42.26%-44.05%（43.45% in average） . The proportion of transition and transversion was 70.30% and 72.70% respectively in codon position 3. The phylogenetic trees were also constructed based on CO I genes of these 8 samples and other 20 Scleractinian species recorded in GenBank. According to the Neighbour- joining, minimum-evolution and maximum-likelihood trees, molecular systematics was slightly different from the morphological conclusion, which suggested that morphological classification may be limited by coral skeletons plasticity. Consequently, the CO I gene is one of the auxiliary tools to classify the phylogeny of Scleractinia.

关 键 词：石珊瑚 线粒体COⅠ 分子系统分类 

分 类 号：Q951.3[生物学—动物学]