农杆菌介导海岛棉胚性愈伤组织遗传转化影响因素分析  

Analysis on Genetic Transformation Affecting Factors of the Agrobacterium-mediated Sea Island Cotton Mbryogenic Callus

作　　者：杨婷[1,2] 曲延英[1,2] 李琼[1,2] 刘艳[1,2] 孟灵真[1,2] 陈全家[1,2] 

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆农业大学农业生物技术重点实验室,乌鲁木齐830052

出　　处：《新疆农业大学学报》2012年第4期302-305,共4页Journal of Xinjiang Agricultural University

基　　金：国家自然科学基金项目(30960201;30700513)

摘　　要：以3个栽培海岛棉品种新海30、新海24、新海16诱导而来的胚性愈伤组织为受体材料,分析了3种农杆菌菌株EHA105、LBA4404、GV3101和3种已构建质粒pCAMBIA1301、pCAMBIA1304、pBI121对已建立的农杆菌介导海岛棉外源基因转化体系的影响。研究结果表明,菌种和质粒对转化率有极其显著的影响,而两者之间的互作关系也比较显著。最终得到农杆菌介导转化海岛棉胚性愈伤组织的最佳菌种和质粒是GV3101和pBI121。This study was conducted to analyze the influence of 3 agrobacterium-mediated plants and 3 plasmids：pCAMBIA1301 ,pCAMBIA1304, pBI121 on the established exogenous gene transformation sys- tem of agrobacterium-mediated sea island cotton, by using the embryogenesis callus of Xinhai 16, Xinhai 24 and Xinhai 30 as receptor materials. The resutls indicated that agrobacterium-mediated and plasmid had significant influence on gentic transformation rate and there were significant mutual relations between the two. Eventually,the best agrobacterium-mediated and plasmid of mbryogenic callus of the sea island cotton were obtained：GV3101 and pBI121.

关 键 词：海岛棉 菌种 质粒 遗传转化 胚性愈伤组织 

分 类 号：S562.032[农业科学—作物学]