皮肤软组织及创伤感染金黄色葡萄球菌分离株的毒素基因检测  

Development of multiplex PCR for detection of pathogenic genes in the Staphylococcus aureus strains isolated from skin and soft tissue infections

作　　者：邓家德[1] 陈惠玲[1] 叶惠芬[1] 凌艳英[1] 李焕庭[1] 杨银梅[1] 周小棉[1] 

机构地区：[1]广州医学院附属广州市第一人民医院检验科,广州510180

出　　处：《中国感染与化疗杂志》2012年第5期368-371,共4页Chinese Journal of Infection and Chemotherapy

基　　金：广东省科技厅资助项目(2011B031800093);广州市医药卫生科技研究基金资助项目(20121A011033)

摘　　要：目的了解皮肤软组织及创伤感染的金葡菌携带的杀白细胞素(Panton—Valentine Leukocidin,PVL)基因、表皮剥脱性毒素(ETs)基因、中毒性休克综合征毒素-1(TSST-1)的tst基因的特点。方法对连续收集的皮肤软组织感染中分离的90株金葡菌,采用多重PCR同时检测金葡菌特异性16SrRNA基因、mecA基因、PVL基因,采用PCR法检测TSST-1及EtsA、B基因。结果金葡菌mecA基因阳性47株(占金葡菌52.2%)为耐甲氧西林金葡菌(MRSA)。有1株MRSA的EtsB基因阳性,1株MRSA的TSST-1阳性,有7株金葡菌携带PVL基因,其中3株为mecA基因阳性株(MRSA)。结论金葡菌可分泌多种毒素,携带PVL毒素的金葡菌常可以引起严重的侵袭性感染,尤其对产毒的MRSA感染引起足够的重视,是防控的重点。Objective To examine the prevalence of the genes encoding Panton-Valentine leukocidin （PVL）, exfoliative toxin （ETs）, toxic shock syndrome toxin 1 （TSST-1）, and some antibiotic resistant genes in the Staphylococcus aureus strains isola- ted from skin and soft tissue infections. Methods A total of 90 strains of S. aureus were isolated from skin and soft tissue infec- tions. Antibiotic susceptibility testing was done by Kirby-Bauer method. The results were interpreted according to CLSI 2009 breakpoints. Multiplex PCR was designed to detect S. aureus specific 16S rRNA gene, rnecA, and PVL gene. The genes enco- ding, TSST-1 and EtsA and EtsB were amplified by PCR. Results The prevalence of mecA gene was 52.2% （47/90）. Positive rnecA defines the strain as methicillin-resistant （MRSA）. EtsB gene was positive in 1 of the MRSA strains. TSST-1 gene was also identified in only 1 MRSA strain. PVL gene was positive in 7 of the S. aureus strains, 3 of which carried mecA gene. Con- clusions S. aureus may release various toxins. PVL-positive strains usually cause severe infection. Attention must be paid to the toxin-producing MRSA strains.

关 键 词：金黄色葡萄球菌 表皮剥脱性毒素 中毒性休克综合征毒素-1 杀白细胞素 基因 

分 类 号：R440[医药卫生—诊断学]