T细胞斑点试验在肠结核诊断的初步探讨  

The primary study of T-SPOT.TB assay in the diagnosis of intestinal tuberculosis

作　　者：邱志兵[1] 钟良[1] 杨海静[1] 戎兰[1] 张文宏 乔春萍[3] 

机构地区：[1]复旦大学附属华山医院消化科,200040 [2]感染科 [3]复旦大学附属华山医院南汇分院

出　　处：《中国感染与化疗杂志》2012年第5期381-383,共3页Chinese Journal of Infection and Chemotherapy

基　　金：国家科技部”国家科技重大新药创制”专项资助(课题编号2008ZX09312-010);上海浦东新区卫生局卫生科技项目(PW2010D-10)资助

摘　　要：目的探讨结核分枝杆菌感染T细胞斑点试验(T-SPOT.TB)对诊断肠结核的临床价值。方法选择13例肠结核患者、20例克罗恩病和20例结肠息肉患者,分别行结核菌素皮试(TST)和T-SPOT.TB检测。结果 13例肠结核、20例克罗恩病和20例结肠息肉患者中,TST阳性分别为7例、3例和3例,T-SPOT.TB阳性分别为12例、5例和4例;TST检测活动性肠结核灵敏度53.8%(7/13),特异度85.0%(34/40)。TST和T-SPOT.TB方法用于检测肠结核灵敏度两者差异有统计学意义(P<0.01)。结论 T-SPOT.TB方法可以作为肠结核诊断的一个有效辅助手段。Objective To evaluate the clinical application of a new enzymelinked immunospot assay （T-SPOT. TB assay） for the diagnosis of tuberculosis. Methods The tuberculin skin test（TST） and T-SPOT. TB assay were performed in blood samples in 13 intestinal tuberculosis patients, 20 the Crohn＇s disease patients and 20 colon polyps patients. Results Among the three kinds of patients, the positive results of TXT were 7 cases, 3 cases and 3 cases, which were 12 cases, 5 cases and 4 cases of T- SPOT. TB. The sensitivity and specificity of TST were 53.85% （7/13） and 85% （34/40）, which were 92.31% （12/13） and 77.5% （31/40） by T-SPOT. TB assay. The significant difference was found （P〈0.01）. Conclusions T-SPOT. TB assay is an effective way for the diagnosis of intestinal tuberculosis.

关 键 词：酶联免疫斑点法 结核菌素皮试 克罗恩病 肠结核 结肠息肉 

分 类 号：R524[医药卫生—内科学]