锦鸡儿属和杜鹃属植物抗乙酰胆碱酯酶活性研究  

Acetylcholinesterase Inhibitory Activity of the Plants of Caragana and Rhododendron

作　　者：杨中铎[1] 任晋[1] 薛鹏辉[1] 

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《中医药学报》2012年第4期67-70,共4页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金(20802031);兰州理工大学优秀青年教师培养计划资助项目(Q200904)

摘　　要：目的:从产自甘肃的锦鸡儿属和杜鹃属植物中筛选出对乙酰胆碱酯酶具有高抑制活性的植物提取物。方法:锦鸡儿属植物经乙醇提取,碱酸处理,乙酸乙酯萃取后,得到多酚提取物。杜鹃属植物经乙醇提取,硅胶柱层析进行粗分后,得到粗分离组分。总多酚组分和粗分离组分采用薄层色谱生物自显影法初步筛选其乙酰胆碱酯酶抑制活性,再用改良的Ellman法进一步测定其对乙酰胆碱酯酶的抑制率。结果:锦鸡儿属中柠条锦鸡儿(Caragana korshinskii)的抑制活性最强,在终浓度为100μg.mL-1时,抑制率超过50%。杜鹃属中,头花杜鹃(Rhododendron capitatum),千里香杜鹃(Rhododendron thymifolium),烈香杜鹃(Rhododendron anthopogonoides)的抑制活性最强,它们的粗分离组分在终浓度为100μg.mL-1时,抑制率大于60%。结论:柠条锦鸡儿(Caragana korshinskii),头花杜鹃(Rhododendron capitatum),千里香杜鹃(Rhododendron thymifolium),烈香杜鹃(Rhododendron anthopogonoides)提取物的抑制活性最强,说明其含有抑制乙酰胆碱酯酶的成分,为进一步跟踪分离得到活性化合物奠定基础。Objective ： To screen acetylcholinesterase inhibitory activity of the plants of Caragana and Rhododendron which were collected from gansu. Methods：The Plants of Caragana were extracted by ethanol and treated with base and hydro- chloric acid, and then extracted by ethyl acetate to obtain the total polyphenol fractions. The Plants of Rhododendron were extracted by ethanol, after a initial separation of silica gel column chromatography, the crude fractions were ob- tained. The AChE inhibitory activity was tested by TLC bioautographic method and modified Ellman＇s colorimetric method in 96 -well microplates. Results： The results indicated that Caragana korshinskii, Rhododendron capitatum, Rhododen- dron thymifolium, Rhododendron anthopogonoides were demonstrated to have significant activity , and their inhibition ra- tio was more than 50% under the concentration of 100 μg · mL-1. Conclusion ： Caragana korshinskii, Rhododendron cap- itatum, Rhododendron thymifolium, Rhododendron anthopogonoides, as sources of AchE inhibitor, are worthy of futher study.

关 键 词：锦鸡儿 杜鹃 阿尔茨海默病 乙酰胆碱酯酶 

分 类 号：R285[医药卫生—中药学]