促溃疡愈合细胞/水凝胶三维复合物的初步构建及表征  

Study on Construction of Accelerating Ulcer Concrescence Cell/Hydrogel Compound and Its Biological Character

作　　者：董荣娜[1] 毋中明[1] 于德民[1] 

机构地区：[1]卫生部激素与发育重点实验室天津医科大学代谢病医院,天津300070

出　　处：《中国医学创新》2012年第26期1-2,共2页Medical Innovation of China

基　　金：中国博士后科学基金项目(编号:20070410766);天津卫生局科技基金项目(编号:07kz47)

摘　　要：目的:应用壳聚糖衍生物水凝胶负载表达人血小板衍化生长因子-BB的CHO细胞系,初步构建促溃疡愈合复合物。方法:选择壳聚糖经N-乙酰-L-半胱氨酸修饰后合成含巯基的双硫键壳聚糖,加水合成水凝胶,用MTT法检测生物相容性并负载转基因CHO细胞,构建促溃疡愈合复合物并进行表征。结果:该水凝胶材料与转基因CHO细胞系生物相容性良好。水凝胶呈网格结构,孔径一致、细胞黏附生长,负载转基因CHO细胞构成三维复合物。结论:CHO-rhPDGF-BB细胞具有新的生物学功能,水凝胶与该细胞相容性良好,为研究糖尿病溃疡创面愈合提供了实验基础。Objective ：Use natural macromolecule hydrogel material to compose 3D cell scaffolds loading transgenic cell that could stably express platelet-derived growth factor-BB （PDGF-BB） to construct accelerating ulcer concrescence compound.Methotl：Choosed chitosan and modified it by NAC to synthesize disulfide bond chitosan with sulfydryl, dissolved the compound into deionized water generating hydrogel, tested it＇s cytotoxicity by MTT ; used it loading transgenic CHO as 3D cell scaffolds to construct cell/scaffold composition, scaned it＇s structure.Result：The hydrogel has good biocompatibility.The compound has network structure, aperture well-distributed, cells stuck well.Conclusion：The CHO cell line had new biological character, and successfully constructed the cell/scaffold composition, which can afford experiment base and theory foundation to study ulcer

关 键 词：PDGF-BB CHO细胞系 糖尿病溃疡 

分 类 号：R632.1[医药卫生—外科学] R587.2[医药卫生—临床医学]