Western印迹法检测IGF-Ⅰ及其受体蛋白在牙鲆仔鱼变态中的表达  

Expression of IGF-Ⅰ and its receptor proteins detected by Western blot during metamorphosis of Paralichthys olivaceus

作　　者：张俊玲[1] 施志仪[1] 翟万营[1] 李巍[2] 

机构地区：[1]上海海洋大学农业部淡水水产种质资源重点实验室,上海201306 [2]全国水产技术推广总站,北京100026

出　　处：《上海海洋大学学报》2012年第5期679-683,共5页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(31172392);上海市重点学科水生生物学建设项目(S30701);上海海洋大学博士启动基金(B-8812-11-0194)

摘　　要：利用Western印迹法对胰岛素样生长因子-Ⅰ(insulin-like growth factor-1,IGF-Ⅰ)及其受体(IGF-Ⅰreceptor,IGF-ⅠR)蛋白在牙鲆仔鱼发育变态阶段的表达进行分析。结果显示IGF-Ⅰ蛋白在仔鱼变态前(17 d)和变态起始阶段(21 d)免疫活性较强,而在变态期间降低;IGF-ⅠR蛋白在变态起始阶段的表达与IGF-Ⅰ类似,但在变态期间与IGF-Ⅰ相反,具有相对丰富的表达。这些结果为进一步研究IGF-Ⅰ及其受体在牙鲆变态中的生理意义奠定了基础。In this study,Western blot was used to analyze the expression of insulin-like growth factor-1（IGF-Ⅰ） and its receptor（IGF-ⅠR） proteins during larval development and metamorphosis of Japanese flounder,Paralichthys olivaceus.The results showed that IGF-Ⅰ protein has a higher immunoreactivity at premetamorphosis（17 d） and the onset of metamorphosis（21 d）,while lower during metamorphosis.The expression pattern of IGF-ⅠR protein at the onset of metamorphosis was similar to that of IGF-Ⅰ,and but an opposite pattern was observed throughout metamorphosis.These results provide a basis for further study on the physiological significance of the IGF-Ⅰ and its receptor in the Japanese flounder metamorphosis.

关 键 词：牙鲆 变态发育 胰岛素样生长因子-Ⅰ 胰岛素样生长因子-Ⅰ受体 WESTERNBLOT 

分 类 号：S917[农业科学—水产科学]