一株高效抑藻放线菌的分离筛选及鉴定  

Isolation and Identification of a High Algicidal Actinomycete

作　　者：郑小伟[1] 黄丽萍[1] 张帮周[1] 张金龙[1] 杨小茹[3] 郑天凌[1,2] 

机构地区：[1]厦门大学生命科学学院,滨海湿地生态系统教育部重点实验室 [2]厦门大学近海海洋环境科学国家重点实验室,福建厦门361005 [3]中国科学院城市环境研究所,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2012年第5期923-928,共6页Journal of Xiamen University：Natural Science

基　　金：国家高技术研究发展计划(863)项目(2008AA09Z408);国家自然科学基金项目(40930847,31070442);近海海洋环境科学国家重点实验室(厦门大学)访问学者基金项目(MELRS1124)

摘　　要：从福建云霄国家红树林自然保护区滩涂沉积物样品中,共分离获得521株纯培养物.通过检测球形棕囊藻(Pha-eocystis globosa)荧光强度计算抑藻率,从521株菌中筛选到27株具有抑藻活性的菌株.在27株抑藻菌中,菌株O3-26对球形棕囊藻具有最高的抑藻率(高达96.71%).菌株O3-26的抑藻谱实验显示,该菌株抑藻活性表现出一定的种属特异性,对硅藻门(Bacillariophyta)的三角褐指藻(Phaeodactylum tricornutum)和中肋骨条藻(Skeletonema costatum)2株测试藻株没有抑制作用,而对绿藻门(Chlorophyta)盐生杜氏藻(Dunaliella salina)和自养小球藻(Chlorella autotrophi-ca)2株藻株具有较强抑藻作用.扫描电镜观察显示,该菌株孢子丝直至螺旋状且孢子表面带刺.生理生化实验显示,该菌株在所得到的大多数培养基上生长良好,在营养琼脂培养基中可以产生水溶性色素;不能在棉子糖作为唯一碳源的培养基上生长.16SrRNA基因相似性分析表明,菌株O3-26属于链霉菌属(Streptomyces),并与灭癌素链霉菌(Streptomycesgancidicus)15412菌株具有最高的同源性(99%).生理生化实验表明,二者之间生理特征存在一定差异.综合形态特征、生理特征以及系统发育分析的结果,鉴定该菌株为灭癌素链霉菌.521 strains were isolated from mangrove sediments in the Yunxiao Mangrove National Nature Reserve,Fujian,China. 27 algicidal isolates were screened based on the analysis of fluorescence intensity of Phaeocystis globosa. The strain named O3-26,which showed the highest algcidal activity （96.71%） against P. globosa among these 27 algicidal isolates, was studied in this article. The test of algicidal spectrum illustrated 03-26 showing a algicidal characteristic of algae species specificity, with high algicidal effects on Dunaliella salina and Chlorella autotrophica , no algicidal effects on Phaeodactylum tricornutum and Skeletonema costatum. Observed by scanning electron microscope,O3 26 has spiral sporothrix and barbed spores. This strain can grow well in most of the medium in this article,and produce soluble pigment in nutrient agar. The physiological tests demonstrated that O3-26 owns a wide range of carbon sources,but it can not be grown on raffinose medium as sole carbon source. On the basis of 16S rRNA gene sequence similari- ty,the strain 03-26 was shown to be related most closely to Streptomyces gancidicus 154152 （99%）. Although some differences from S. gancidicus were tested from physiological and biochemical experiments,O3-26 was still indentificated as S. gancidicus by comparison on morphological,physiological characteristics and phylogenetic analysis.

关 键 词：赤潮 球形棕囊藻 抑藻放线菌 灭癌素链霉菌 

分 类 号：Q939[生物学—微生物学]