东亚飞蝗TLR9基因cDNA克隆及蛋白质序列分析  

Cloning and Sequence of Toll-like Receptor 9 Gene of Locus ta migratoria manilensis

作　　者：刘迎春[1] 聂惠蓉[1] 李裕红[2] 

机构地区：[1]福建医科大学细胞生物学与遗传学系,福建福州350004 [2]泉州师范学院生物系,福建泉州362000

出　　处：《长江大学学报（自科版）（中旬）》2012年第6期18-21,28,共5页Journal of Yangtze University(Nature Science Edition)

基　　金：福建省自然科学基金项目(2009J05068);福建医科大学博士启动基金项目(2009BS002)

摘　　要：东亚飞蝗(Locusta migruoria manilensis)依靠先天性免疫系统来防御病原微生物的入侵,TLRs是天然免疫重要的病原体识别受体。在已知的东亚飞蝗TLR9受体基因(LmTLR9)的cDNA部分序列基础上,进一步获得其序列并对其序列进行分析;观察金龟子绿僵菌(Metarhizium anisopliae)的侵染对蝗虫LmT上R9 mRNA表达的影响。结果表明获得的LmTLR9 cDNA序列含有1个2340bp的开放阅读框,推定的蛋白质序列由780个氨基酸残基组成。与黑腹果蝇、冈比亚按蚊和致倦库蚊的氨基酸序列同源性分别为19.0%、26.0%和26.0%;与对照组相比,侵染绿僵菌24h和48h后,试验组东亚飞蝗中肠TLR9 mRNA分别升高了7.17%和4.97%,但无显著性差异。Locusta migratoria manilensis depends on innate immune system against pathogen invasion.In the mediation of the systemic effects of pathogens,Toll-like receptors (TLRs) play a significant role.TLRs are innate immune receptors that are critical in the host defense against many types of invading pathogens.The gene LmTLR9(toll-like receptor 9 from Locusta migratoria manilensis )was obtained and analyzed,and the expressions of LmTLR9 mRNA after the invasion of Metarhizium anisopliae were detected. The results showed that the locust TLR9 cDNA was 2340bp in length with an open reading frame encoding 780 amino acids residues.The amino acids sequence shared 19.0%,26.0%and 26.0%homology with that of Drosophila melanogaster,Anopheles gambiae and Culex quinquefasciatus,respectively.The structure prediction showed that the locust TLR9 is a typical typeⅠtransmembrane protein composed of extracellular, transmembrane and intracellular regions,which contain leucine-rich repeats(LRR) ectodomains and Toll/IL-1R domain(TIR) cytoplsmic domains.The expression levels of LmTLR9 mRNA were examined by fluorogenic quantitative RT-PCR on 0,24h and 48h after the invasion of M.anisopliae.The expression levels of LmTLR9 mRNA of group 24h and 48h has increased 7.17%and 4.97%,respectively.These results indicated that the locust TLR9 could play a crucial role in recognition of pathogen-associated molecular patterns and signal transduction.However,LmTLR9 may not involve in the host response to a fungal infectious pathogen,M.anisopliae.

关 键 词：东亚飞蝗(Locustamigratoria manilensis) Toll样受体9基因 序列分析 

分 类 号：Q78[生物学—分子生物学]