SSR鉴定水稻两系杂交种纯度的运用与探讨Ⅱ.不同苗龄取样方法对室内纯度结果的影响  

Study on Detection of Seed Purity of Two-line Hybrid Rice by Simple Sequence Repeat (SSR)Ⅱ.Effects of Sampling Methods on Laboratory Purity Detection

作　　者：廖芳丽 张宇飞 林梅 赵虹 熊先锋 涂书新[2] 张凯[3] 

机构地区：[1]荆州市种子管理局,湖北荆州434020 [2]华中农业大学,武汉430070 [3]长江大学农学院,湖北荆州434025

出　　处：《湖北农业科学》2012年第17期3687-3689,共3页Hubei Agricultural Sciences

基　　金：农业部公益性行业科研专项(201103007)

摘　　要：以水稻(Oryza sativa L.)两优17为试验材料进行标准发芽后,对幼苗进行大苗、小苗分类,并利用SSR引物分别对其进行室内纯度鉴定。鉴定结果表明,苗的大小对鉴定结果的影响较大,小苗与大苗的纯度相差2.8个百分点,超出了允许的误差范围。与大田相比,大苗的纯度更接近于田间秧苗纯度。在田间小苗往往不能成苗,不影响大田纯度,故小苗是造成室内与大田纯度鉴定结果产生差异的重要原因之一。Seedlings of Liangyou17 were obtained from standard germination and classified as large or small seedlings.The laboratory seed purity was tested by SSR.The results showed that the size of seedlings had obvious effect on identification of purity as the difference between large and small seedling was 2.8%,which exceed the allowable error range.The purity of large seedling was similar with that of field.Meanwhile,the small seedlings had limited effect on the purity of field as they could not mature thus was the key factor contribute to the difference between seeds purity in laboratory and field.

关 键 词：水稻(Oryza SATIVA L.) SSR 室内 田间 纯度鉴定 

分 类 号：Q789[生物学—分子生物学] S511[农业科学—作物学]