色木槭芽直接增殖途径的培养条件  

The tissue culture system for microshoot proliferation through bud organgenesis of Acer mono Maxim

作　　者：杨玲[1] 王娜[1] 施志娟[1] 马欢[1] 官志玉[1] 沈海龙[1] 

机构地区：[1]东北林业大学林学院,林木遗传育种国家重点实验室,哈尔滨150040

出　　处：《林业科技开发》2012年第5期67-71,共5页China Forestry Science and Technology

基　　金：国家大学生创新性实验项目(编号:1110225001);黑龙江省教育厅科学技术研究指导项目(编号:11553034);中央高校基本科研业务费专项资金(编号:DL10BA04)

摘　　要：为建立色木槭芽直接增殖的诱导培养体系,以色木槭野生树木休眠芽萌发枝条为材料,进行芽增殖的培养条件研究。结果表明,4月份是适宜休眠芽培养的取材时期。嫩茎在2%次氯酸钠浸泡20 min是最佳消毒方案。NAA浓度对休眠芽萌发和嫩茎生长的影响具有显著差异。MS+0.1 mg/L NAA+20 g/L蔗糖(pH5.8)是适合休眠芽萌发和嫩茎伸长的培养基。培养30天时,腋芽萌发率可达63.3%,嫩茎平均高度可达15.9 mm。6-BA对芽直接增殖的促进效果好于KT。不同激素组合中,IBA与6-BA组合对芽增殖和丛生芽生长的效果好于NAA与6-BA组合、NAA与KT组合、IBA与KT组合。MS+0.1 mg/L IBA+1 mg/L 6-BA+20 g/L蔗糖是适合芽增殖和丛生芽生长的培养基。培养30天时,芽增殖率可达90%,增殖倍数可达3.19,且茎芽生长正常。Culture conditions for shoot proliferation from sproutings of dormant axillary buds collected on field trees of Acer mono Maxim were checked for the purpose of establishing a direct shoot proliferation system. The results showed that April was the most suitable periods for collecting dormant buds; the maximum disinfection method for sproutings from dormant buds was souking in 2% sodium hypochlorite （NaClO） for 20 min; NAA concentrations had significant effect on dormant bud sprouting and the growth of the sproutings; MS ＋ 0. 1 mg/L NAA ＋ 20 g/L sucrose （ pH = 5.8 before autocalving） was the suitable medium for dormant bud sprouting and the growth of the sproutings;the sprouting rate of axillary buds was 63.3% and the average height of sproutings was 15.9 mm at the 30 days of culture; 6-BA was better than KT for direct shoot multiplication; the combination of IBA and 6-BA was better than that of NAA and 6-BA, NAA and KT, IBA and KT for proliferation and growth of the in vitro culture shoots ; the best suitable medium for proliferation and growth of the in vitro cultured shoots was MS ＋ 0. 1 mg/L IBA ＋ 1 mg/L 6-BA ＋ 20 g/L sucrose, the proliferation rate and fold could reach 90% and 3.19 respectively, and the shoots grew well at the 30 days of culture.

关 键 词：植物生长调节剂 色木槭 芽增殖 丛生芽诱导 

分 类 号：S792.119[农业科学—林木遗传育种]