丹参酸提液对高脂大鼠骨量的影响  

The effects of acid extract of salvia miltiorrhiza Bge on bone mass of high-lipid rats

作　　者：张新乐[1] 吴铁[1] 崔燎[1] 许碧莲[1] 邹丽宜[1] 刘钰瑜[1] 陈文双[1] 

机构地区：[1]广东医学院药理教研室,广东湛江524023

出　　处：《广东医学》2012年第17期2535-2538,共4页Guangdong Medical Journal

基　　金：2011年广东省第一批科学事业费计划项目(编号:2011B031800248)

摘　　要：目的观察丹参酸提液对高脂大鼠胫骨和腰椎骨丢失的防治作用。方法选取3月龄SD大鼠30只,随机分为正常对照组(NS组)、高脂乳剂组(HFE组)、丹参酸提液组(SMB组),每组10只。NS组灌胃生理盐水,HFE组和SMB组灌胃高脂乳剂建立高脂血症模型,SMB组同时给予丹参酸提液。给药16周后处死大鼠,分离血清检测胆固醇、三酰甘油和高密度脂蛋白胆固醇(HDL-C),并取胫骨上段、胫骨中段和第5腰椎,进行骨组织形态计量学参数检测。结果与HFE组比较,SMB组大鼠HDL-C升高(P<0.05),骨小梁面积百分数、骨小梁数量、成骨细胞贴壁长度百分率均增加(P<0.05),而骨小梁分离度、每毫米破骨细胞数、破骨细胞贴壁长度百分率均减少(P<0.01);胫骨中段的皮质骨面积百分数增加(P<0.01),骨髓腔面积百分数减少(P<0.01);腰椎松质骨的骨小梁面积百分数、骨小梁宽度增加(P<0.05),而骨小梁分离度减少(P<0.01)。结论长期高脂乳剂灌胃会导致大鼠骨量丢失,丹参酸提液能有效对抗高脂大鼠胫骨上段、中段骨、第5腰椎的骨丢失。Objective To study the effects of acid extract of Salvia mihiorrhiza Bge （SMB） on the prevention of bone mass loss in tibia and vertebrae in high - fat - induced osteopenia rats. Methods Thirty 3 - month - old male Spra- gue- Dawley （SD） rats were randomly divided into NS group, high- fat emulsion （HFE） group, and SMB group. High fat emulsion lavage was given to the rats in HFE and SMB groups for construction of high - fat rat model, while stimulatory SMB extract was given to rats in SMB group. The subjects were sacrificed at 16th week for assessment of serum TC, TG, and HDL- C. PTM, LVB and Tx sections were collected for histomorphometric analysis. Results Compared with the HFE group, there were significant increase of serum I-IDL - C, % Tb. Ar, Tb. N and % Ob. S. Pm, while significant reduc- tion in Tb. Sp, % Oc. S. Pm and Oc. N/mm were also revealed in SMB group （P 〈 0. 05）. Meanwhile, there was signifi- cant increase of % Ct. Ar with reduction of % Ma. Ar in Tx of SMB group （ P 〈 0. 05 ） ; so were the increase of % Tb. Ar and Tb. Th and reduction of Tb. Sp in LVB （ P 〈 0. 05 ）. Conclusion SMB can effectively reverse the long - term high - fat diet -induced bone loss in rats.

关 键 词：高脂乳剂 丹参酸提液 骨质疏松 骨组织形态计量学 大鼠 

分 类 号：R285.5[医药卫生—中药学]