增强表达PTEN蛋白对类风湿关节炎成纤维样滑膜细胞增殖的作用  

The effects of PTEN expression on reproduction of FLS in rheumatoid arthritis

作　　者：李曼[1] 王栋[1] 徐宁[1] 黄宪章[1] 庄俊华[1] 黎小琼[1] 

机构地区：[1]广东省中医院医学检验部,广州510370

出　　处：《广东医学》2012年第17期2539-2541,共3页Guangdong Medical Journal

基　　金：广东省自然科学基金资助项目(编号:10151040701000031);广东省中医院拔尖人才基金资助项目(编号:2011-84)

摘　　要：目的研究增强表达PTEN蛋白对类风湿关节炎成纤维样滑膜细胞(RA FLS)增殖的影响。方法慢病毒表达载体pLenti6/V5-PTEN转染RA FLS并表达PTEN蛋白,应用RT-PCR、Western blot检测PTEN表达,MTT法检测RA FLS增殖能力的变化。结果转染pLenti6/V5-PTEN后,RA FLS中PTEN mRNA表达提高60%以上,PTEN蛋白表达在48 h后提高了107.85%(P<0.05),RA FLS增殖在487、29、6 h分别下降了25.14%、41.84%和52.46%(P<0.05)。结论慢病毒表达载体pLenti6/V5-PTEN能够提高PTEN蛋白表达水平,并显著抑制RA FLS增殖。Objective To study the effects of PTEN expression on the reproduction of FLS in rheumatoid arthritis （RA）. Methods FLS were transfected with pLenti6/V5 - PTEN for over - expression of PTEN. Subsequently, the ex- pression of PTEN was assessed with RT - PCR and Western Blot. The proliferation of FLS was also assessed. Results After transfected with pLenti6/V5 - PTEN, the expression of PTEN mRNA and protein in FLS were significantly increased by 63% and 107. 85% , respectively （P 〈0. 05）. At the same time the proliferation of FLS was significantly reduced by 25.14% , 41.84% and 52.46% at 48 h, 72 h and 96 h, respectively. Conclusion Lentivirus vector pLenti6/V5 - PTEN can enhance PTEN expression, thus effectively inhibits FLS proliferation in RA.

关 键 词：PTEN 慢病毒表达载体 类风湿关节炎 

分 类 号：R593.22[医药卫生—内科学]