光敏剂SiPc(C_(35)H_(27)O_5)_8介导的PDT对人RPE的影响  

Influence of photosensitizer SiPc(C_(35)H_(27)O_5)_8 mediated PDT on retinal pigment epithelial cell

作　　者：徐国兴[1] 周晓芳[1] 陈鲤敏[1] 彭亦茹[2] 

机构地区：[1]福建医科大学附属第一医院眼科福建省眼科研究所,中国福建省福州市350005 [2]福建师范大学,中国福建省福州市350007

出　　处：《国际眼科杂志》2012年第10期1841-1843,共3页International Eye Science

基　　金：国家自然科学基金课题(No.81070715);中国卫生部科研基金课题(No.WKJ2008-2-61);福建省创新平台基金课题(No.2010Y2003)~~

摘　　要：目的:探讨SiPc(C35H27O5)8介导的PDT对人RPE影响的可能机制。方法:将hRPE细胞分空白对照组、激光组、光敏剂SiPc(C35H27O5)8组、PDT1J组、PDT2J组,对各实验组应用CCK-8、荧光显微镜蓝光激发、DCFH-DA检测RPE细胞线粒体活性氧水平与RPE细胞凋亡率。结果:不同浓度光敏剂[SiPc(C35H27O5)8]介导的PDT对RPE产生的作用不一样,其中以5μg/L的浓度对RPE的杀伤作用最大。结论:新型光敏剂SiPc(C35H27O5)8介导的PDT对RPE具有一定的杀伤作用,其可能机制为光敏剂[SiPc(C35H27O5)8]介导的PDT对RPE细胞线粒体活性氧水平增加导致细胞凋亡。AIM： To study the mechanism of SiPc（C35H27O5）8 mediated PDT on retinal pigment epithelial cell（RPE）. METHODS： hRPE cells were divided into blank control group, laser group, photosensitizer SiPc（C35H27O5）8 group, PDT 1J group, PDT 2J group. CCK-8, fluorescent microscopes blue excitation were applied in the experimental groups, and active oxygen level of RPE cell mitochondria and RPE cell apoptosis rate were detected by DCFH-DA. RESULTS： Different concentration photosensitizer ／〖SiPc（C35H27O5）8／〗 mediated PDT on RPE had different effects, in which 5 μg/L of concentration had the most serious damage. CONCLUSION：New photosensitizer SiPc（C35H27O5）8 mediated PDT on RPE has certain damage effect, its possible mechanism is that the increasing RPE cell mitochondria reactive oxygen species level caused by photosensitizer ／〖SiPc（C35H27O5）8／〗 mediated PDT results in cell apoptosis.

关 键 词：酞菁硅 荧光 光动力学 凋亡 流式细胞术 

分 类 号：R454[医药卫生—治疗学]