补中益气汤对脾虚大鼠胃粘膜MUC5ACmRNA与蛋白表达的影响  

Effect of Buzhongyiqi Decoction on the expression of gastric mucosa MUC5ACmRNA and protein in rats with the syndrome of Spleen Deficiency(脾虚)

作　　者：刘晓玲[1] 王汝俊[1] 赵琪琪[1] 

机构地区：[1]广州中医药大学,广州510006

出　　处：《中药药理与临床》2012年第4期7-9,共3页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:观察脾虚大鼠胃粘膜MUC5ACmRNA与蛋白表达的变化及补中益气汤对其表达的影响,并探讨相关作用机制。方法:SD大鼠称重随机分为空白对照组、脾虚损伤组、补中益气汤组、非脾虚损伤组。脾虚损伤组、补中益气汤组大鼠给予100%大黄水煎剂灌胃,一天2次,连续10天。第11天补中益气汤组给予补中益气汤灌胃治疗7天,第18天,除空白对照组外的另外三组均灌服消炎痛造成胃粘膜损伤。采用逆转录PCR(RT-PCR)检测MUC5ACmRNA,用双抗体两步夹心酶联免疫吸附法(ELISA)检测MUC5AC蛋白。结果:与非脾虚损伤组比较,脾虚损伤组大鼠胃粘膜MUC5AmRNA、蛋白表达有所下降,但无统计学意义;与脾虚损伤组比较,补中益气汤组大鼠胃粘膜MUC5AC蛋白表达显著升高。结论:脾虚大鼠消炎痛攻击后胃粘膜MUC5AC蛋白表达显著下调,提示脾虚时胃粘液凝胶层防御能力下降,补中益气汤可提高脾虚大鼠MUC5AC蛋白的表达,提示该方可通过粘液蛋白MUC5AC促进胃粘液凝胶层的形成,增加胃粘液屏障防护功能,并与TFF1发挥协同增效作用,这可能是补中益气汤胃粘膜保护的机制之一。Object： To observed the expression of gastric mucosa MUCSACmRNA and protein in the Spleen Deficient and the effect of Buzbongyiqitang, the related mechanisms were discussed. Methods： The rats were randomly divided into the spleen deficiency with injury group, The model of spleen deficiency was copied by Dahuang, and Buzhongyiqitang group , the blank control group, and the non- spleen deficiency with injury group according to their weight. The spleen deficiency with injury group and the Buzhongyiqitang group were administrated by 100% DaHuang solution, twice a day , for 10 days. Then the Buzhongyiqitang group was treated by Buzhongyiqitang for 7 days, twice a day. In ad- dition to the control group, the other three groups were fed with indomethacin solution to cause mucosa injruy after treatment. RT-PCR was used to assay MUC5ACmRNA, ELISA was used to detect the expression of MUC5AC protein. Results： Compared with the non-spleen defi- ciency with injury group, the expression of gastric mucosa MUC5ACmRNA and protein in the spleen deficiency with injury group were de- creased in some degree（ P 〉 0.05 ） ; Compared with the spleen deficiency with injury group, the expression of gastric mucosa MUC5AC pro- tein in the Buzhongyiqitang group was increased significantly（ P 〈0.05）. Conclusion： The expression of gastric mucosa MUCSAC protein in the spleen deficiency with injury group was decreased significantly, suggesting the decreasing mucosal defense capabilities in rats with spleen deficiency. Buzhongyiqitang increased the expression of MUCSAC protein greatly, to promote the formation of gastric mueosal gellayer. MUC5AC was bound to TFF1, which may be one of gastric mucosa＇s protective mechanisms of Buzhongyiqitang.

关 键 词：补中益气汤 脾虚大鼠 MUC5AC 

分 类 号：R285[医药卫生—中药学]