二氢杨梅素对醛糖还原酶的抑制作用  

Inhibitory effect of dihydromyricetin on aldose reductase activity

作　　者：刘恺[1] 高丽辉[1] 牛艳芬[1] 袁丽仙[1] 刘旭[1] 李玲[1] 

机构地区：[1]昆明医学院生物医学工程研究中心,昆明650500

出　　处：《中药药理与临床》2012年第4期43-45,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金资助项目(30760306);云南省应用基础研究计划重点项目(2006C009Z)

摘　　要：目的:研究二氢杨梅素体外对醛糖还原酶(AR)的影响。方法:取正常SD大鼠晶状体制备醛糖还原酶,以依帕司他1×10-7mol/L为阳性对照,观察二氢杨梅素1.0×10-5、4.0×10-5、1.4×10-4、6.4×10-4mol/L四个浓度体外对醛糖还原酶的抑制作用。结果:随着二氢杨梅素浓度的增加,醛糖还原酶的活性逐渐降低,且呈一定的剂量-效应关系,在终浓度为6.4×10-4mol/L时,其对醛糖还原酶活性的抑制率为54.3%。结论:二氢杨梅素在体外有抑制醛糖还原酶的作用。Objective： To study the effect of dihydromyricetin on the activity of aldose reductase （AR） in vitro. Methods： Aldose reductase was ex- tracted from lens of normal SD rat. Epalretat （ 1×10^-7 mol/L） was as a positive. The inhibitory effects of dihydromyricetin, at a concentra- tion of 1.0 × 10^-5 , 4.0 × 10^-5 , 1.4 ×10^4 and 6.4 ×10^4 tool/L, on the activity of aldose reductase were investigated in vitro. Results： Di- hydromyricetin markedly inhibited the activity of aldose reductase, in a concentration-dependent manner, with the inhibitory ratio of 54.3% at the concentration of 6.4×10^-4 mol/L. Conclusion： Dihydromyricetin has the inhibitory activity on aldose reduc＇tase in vitro.

关 键 词：醛糖还原酶 二氢杨梅素 糖尿病 

分 类 号：R285[医药卫生—中药学]