健脾益气方对实验性重症肌无力小鼠FoxP3和TGF-β1的干预研究  

Study of Jianpiyiqi Decoction on EAMG mice of FoxP3 and TGF-β1

作　　者：董婷[1] 杨文明[1] 王晓旸[1] 汪瀚[1] 汪美霞[1] 韩辉[1] 张娟[1] 李静[2] 黄鹏[2] 

机构地区：[1]安徽中医学院第一附属医院,合肥230031 [2]安徽中医学院,合肥230031

出　　处：《中药药理与临床》2012年第4期89-91,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：安徽省高等学校省级自然科学基金(项目编号:KJ2011Z221)

摘　　要：目的:观察健脾益气方对EAMG小鼠FoxP3和TGF-β1调节作用。方法:采用N2AchR加等量福氏完全佐剂,分2次免疫C57BL/6小鼠,并复制重症肌无力(experimental auto-immune myasthenia gravis,EAMG)模型,及对模型进行评价。小鼠随机分为佐剂组、模型组、地塞米松组和健脾益气方组,采用ELISA法观察各组动物血清AChR-Ab水平和TGF-β1水平,实时荧光定量聚合酶链反应(RT-FQ-PCR)分析4组小鼠脾细胞中Foxp3mRNA的表达。结果:健脾益气方组(起效剂量18.8g/kg,剂量范围18.8g/kg～37.6g/kg)、地塞米松组小鼠血清AchR-Ab滴度明显降低,与模型组和佐剂组比较有非常显著性差异,但两治疗组之间无显著性差异。结论:通过降低FOXP3mRNA、TGF-β1的表达,可能增加AChR抗体的产生,在MG的发病中起到重要作用;中药健脾益气方具有良好的防治EAMG效果,可能是通过上调TGF-β1和FoxP3水平,抑制AchR-Ab产生而起到治疗作用。Objective ：To observe the regulating action of Jianpiyiqi Decoction （JPYQ） on experimental auto-immune myasthenia gravis （EAMG） mice of FoxP3 and TGF-β1. The C57BL/6 mice were twice immunized with N2AchR emulsified in an equal volume of complete Freund;s adju- vant and EAMG model were duplicated as well as evaluated. Mice were randomly divided into adjuvant group, model group, hexadecadrol group ,and Jianpiyiqi Decoction group. To examine the levels of AChR-Ab and TGF-β1 with enzyme linked immunosorbent assay,analyse the expression of Foxp3mRNA in the quadruplet mice splenocyte with RT-FQ-PCR. Result： Compared with model group and adjuvant group, the serum level of anti-Ach R antibodies （AchR-Ab） of Jianpiyiqi Decoction group （ effect dose： 18.8g/kg, dose range： 18.8g/kg - 37.6g/kg） and bexadecadrol group was deseased,showing a significant difference（ P 〈 0.01 ）. There was no obvious difference between two treatment groups（ P 〉 0.05 ）. Conclusion： Anti-AchR may be increased by reducing the expression of FOXP3mRNA and TGF-β1 which plays an important role in the etiology of MG. JPYQ can nicely prevent and treat EAMG probably due to suppress AchR-Ab by enhancing the level of TGF-β1 and FoxP3.

关 键 词：健脾益气方 重症肌无力小鼠 FOXP3 TGF-Β1 AChR-Ab. 

分 类 号：R285[医药卫生—中药学]