猪繁殖与呼吸综合征病毒通过降解IκBα激活NF-κB信号通路  

Porcine reproductive and respiratory syndrome virus activated the NF-κB pathway through IκBα degradation

作　　者：康艳梅[1] 裴晶晶[1] 王佳莹[1] 赵明秋[1] 刘文俊[1] 陈金顶[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《中国预防兽医学报》2012年第10期770-773,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：公益性行业(农业)科研专项经费(201203056);国家自然科学基金(31072137;31172321);广东省自然科学基金重点项目(NO.S2011020001037)

摘　　要：核转录因子κB(NF-κB)在调节细胞免疫功能及细胞增殖和存活方面均发挥着重要的作用。本研究采用高致病性猪繁殖与呼吸综合症病毒(HP-PRRSV)GD08-1株和经典株PRRSV GD-XH分别感染Marc-145细胞,于感染后不同时间提取胞浆蛋白和核蛋白,通过电泳迁移率(EMSA)检测NF-κB与DNA结合活性及western blot检测胞浆蛋白中NF-κB及其抑制因子(IκB)的表达情况。HP-PRRSV株和PRRSV经典株感染Marc-145核蛋白的EMSA结果中均出现NF-κB与DNA探针的结合条带;western blot结果表明,HP-PRRSV株和PRRSV经典株感染Marc-145细胞并未引起NF-κB表达量的变化,但两者均引起了IκB表达水平的下降,由此推测,HP-PRRSV株和经典PRRSV株感染均能够刺激Marc-145细胞NF-κB活化入核内,PRRSV感染Marc-145细胞引起的NF-κB的活化是通过IκB的降解来实现的。Nuclear factor-kappaB （NF-κB） is a critical regulator of immune function as well as cell proliferation and survival. In this study, Marc-145 cells were infected with highly pathogenic pocine reproductive and respiratory syndrome virus （HP-PRRSV） GD08-1 strain and PRRSV GD-XH strain. The cytoplasmic and nuclear protein were extracted from Marc-145 cells at different post infection, respectively. DNA binding activity of NF-κB was assessed by EMSA and the protein expressing levels of NF-κB and IKBα in cytoplasmic from Marc-145 cells was assessed by western blot. The results indicated that both NF-κB in nuclear proteins from HP-PRRSV and PRRSV infected Marc-145 cells were positively binding to DNA. However, the cytoplasmic protein levels of both NF-κB had no changed in virus infected Marc-145 cells, but IKBα protein levels were degraded in both viruses infected cells. These results demonstrated that both HP-PRRSV and PRRSV infection resulted in an increase in NF-κB nuclear translocation and NF-κB-DNA binding activity mediated by IKBα degradation in MARC-145 cells.

关 键 词：猪繁殖与呼吸综合征病毒 NF-ΚB MARC-145 IΚB 信号通路 

分 类 号：S852.65[农业科学—基础兽医学]