广东珠三角地区犬细小病毒分离与鉴定  

Isolation and identification of canine parvovirus in Pearl River Delta region of Guangdong

作　　者：黄永亮[1] 施赫赫[1] 冯顺意[1] 王怡飞[1] 米政实[1] 刘慧思[1] 徐晓娟[1] 张莹[1] 吴晓薇[1,2] 牛学锋[1,3] 郭霄峰[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]广东省出入境检验检疫技术中心,广东广州510642 [3]Department of Veterinary Pathology, Athens, GA 30602

出　　处：《中国预防兽医学报》2012年第10期778-781,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：2007年广东省动物防疫检疫研究专项(粤财农[2007]479号)

摘　　要：为了解珠三角地区犬细小病毒(CPV)的流行情况及研制有效的疫苗,本研究开展了CPV流行病学调查,并将32份疑似CPV感染犬的粪便样品处理后接种F81细胞进行病毒分离,并对分离株进行鉴定。鉴定结果显示:32份样品中有19份样品提取液在F81细胞上可以产生明显的细胞病变;HA效价可达到1∶256。回归动物试验显示分离株可以导致犬出现明显的CPV感染症状,PCR鉴定也进一步确定所分离的病毒为CPV。通过VP2基因同源性分析显示分离株与国内主要的流行基因亚型CPV-2a的同源性达98%以上。An epidemiological survey for canine parvovirus （CPV） was conducted in Pearl River Delta region and faece samples from infected dogs were collected for virus isolation. The clinical samples were examined by PCR, immunofluorescent assay, hemagglutination test, and the results showed that 19 CPV isolates were isolated from 32 samples. The sequence analysis indicated the nucleotide sequence homology was above 98% with CPV-2a. The results from this molecular investigation of CPV show the prevalence of type 2a was the main type CPV in pearl river delta regio.

关 键 词：犬细小病毒 分离 鉴定 

分 类 号：S852.65[农业科学—基础兽医学]