秀丽隐杆线虫的培养条件及其在捕食线虫性真菌研究中的应用  

Culture conditions of Caenorhabditis elegans and its application in the study of nematode-trapping fungi

作　　者：王俊伟[1] 孟庆玲[1] 乔军[1] 才学鹏[2] 罗建勋[2] 王为升[1] 张再超[1] 蔡扩军[1] 

机构地区：[1]石河子大学动物科技学院预防兽医学重点实验室,新疆石河子832003 [2]中国农业科学院兰州兽医研究所、家畜疫病病原生物学国家重点实验室/甘肃省动物寄生虫病重点实验室,甘肃兰州730046

出　　处：《中国预防兽医学报》2012年第10期797-801,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：兵团博士资金(2009JC09);石河子大学高层次人才专项(RCZX200901)

摘　　要：为进一步了解模式线虫-秀丽隐杆线虫(C.elegans),并为筛选高效力的家畜寄生线虫生防真菌提供实验依据,本研究通过对C.elegans的形态、培养条件以及保存方法的研究,发现C.elegans在16℃～25℃NGM培养基中均可以生长,但在20℃NGM培养基中生长较佳。在-80℃或液氮的温度条件下,C.elegans在30%甘油溶液和SA冻存液中均可以保存至少2年。同时,本研究探索了不同龄期C.elegans幼虫对供试菌-少孢节丛孢菌新疆分离株(Arthrobotrys oligospora XJ-XA)的捕食器官的诱导能力,并测定了供试菌株对不同龄期C.elegans幼虫的捕食率,分析了菌株捕食器官产生、捕食能力与线虫龄期的关系。结果表明,不同龄期C.elegans对供试菌的捕食器官诱导产生的数量有明显差异,而且供试菌对不同龄期C.elegans的捕食能力也存在差异。Caenorhabditis elegans is an important model nematode for modem developmental biology, genetics and genomics etc. It was found that C. elegans was able to grow at 16℃ to 25 ℃ in NGM medium. However, it was better to grow at 20 ℃ from the morphology, culture condition and preservation of C. elegans. And under the condition of -80 ℃ or liquid nitrogen, stocks were survived for at least 2 years in 30% glycerol solution or SA frozen solution. Meanwhile, the induction ability of different larvae of C. elegans to A. oligospora XJ-XA was explored with the detection of trapping rate of predation of the same fungus to different larvae, and the relationship was analyzed between the production of trapping device, predation ability and juvenile stages of nematode. The results demonstrated that there was a significant difference in the induced amount of the capturing device of the predating strain, and there were also great differences in the predating ability of the same strain to different larvae.

关 键 词：秀丽隐杆线虫 培养 保存 少孢节丛孢菌 捕食器官 

分 类 号：S852.7[农业科学—基础兽医学]