Study on Mutant Induction of Gladiolus by in vitro Culture of Petals  

作　　者：Li Cai-hua Fan Jin-ping Gong Shu-fang Che Dai-di 

机构地区：[1]College of Horticulture, Northeast Agricultural University, Harbin 150030, China [2]Economic Crops Research Institute, Heilongjiang Land Reclamation Academy of Science, Harbin 150086, China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2012年第3期38-42,共5页东北农业大学学报（英文版）

基　　金：Supported by Heilongjiang Province Science and Technology Research Projects (GB06B112-5)

摘　　要：Petals of Gladiolus ＇Rose Supreme＇ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in Mj generation by ISSR molecular markers and traits were observed in M3 generation. The results showed that the best medium to induce callus was MS＋2, 4-D 4.0 rag＂ L-~＋6-BA 0.5 rag. L~ and the best medium to induce somatic embryogenesis was MS＋2, 4-D1.0 mg＇L-I＋TDZ0.3 mg-L-1. New petals could be formed from petal callus directly. Pigments of mother plant appeared on plantlets while they were formed from petals. Two mutants were obtained from petal somaclonal line in M3 generation which demonstrated the correctness of ISSR analysis in M1 generation.Petals of Gladiolus ＇Rose Supreme＇ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in Mj generation by ISSR molecular markers and traits were observed in M3 generation. The results showed that the best medium to induce callus was MS＋2, 4-D 4.0 rag＂ L-~＋6-BA 0.5 rag. L~ and the best medium to induce somatic embryogenesis was MS＋2, 4-D1.0 mg＇L-I＋TDZ0.3 mg-L-1. New petals could be formed from petal callus directly. Pigments of mother plant appeared on plantlets while they were formed from petals. Two mutants were obtained from petal somaclonal line in M3 generation which demonstrated the correctness of ISSR analysis in M1 generation.

关 键 词：GLADIOLUS PETAL VARIATION 

分 类 号：S682.24[农业科学—观赏园艺] Q943.1[农业科学—园艺学]