高活性辣根过氧化物酶-金纳米偶联物的制备与表征  

Preparation and Identification of Highly Active Horseradish Peroxidase-gold Nanoparticle Conjugate

作　　者：王丽英 刘春丽 张鹏[1] 彭池方 

机构地区：[1]江南大学食品科技学院,江苏无锡214122

出　　处：《纳米科技》2012年第4期27-31,共5页

基　　金：教育部博士点新教师基金项目（No.20110093120003）

摘　　要：以辣根过氧化物酶（HRP）为模型，研究了一种新型的将蛋白分子固定在金纳米粒子（GNP）表面的方法，制备得到一种高活性的辣根过氧化物酶-金纳米粒子生物偶联物。采用水溶性碳化二亚胺法制备了生物素化辣根过氧化物酶在柠檬酸根离子稳定化的金纳米粒子表面，通过静电吸附链霉亲和素。生物素-亲和素体系方便地制备得到高活性的HRP—GNP生物偶联物，单位酶活力提高了10倍以上。HRP—GNP生物偶联方法在制备高活性的生物探针和固定化酶等领域具有广泛的应用前景。Taking horse radish peroxide enzyme （HRP） as the model, a novel method of immobilizing protein onto the surface of gold nanoparticles （GNP） was developed, and HRP-GNP, a high-activity bionanoconjugates was prepared. Bi- otinylated HRP was prepared by water-soluble carbodiimide method. On the surface of citrate ion stabilized gold nanoparticles, streptavidin was adsorbed through electrostatic interaction. The HRP-GNP conjugated with high activity was conveniently prepared through biotin-avidin system, the unit enzyme activity of this new conjugate was enhanced by 10 times. Therefore,the method of HRP-GNP bioconjugation preparing method has promising applications fields in preparing highly active bio-nanoprobe and immobilized enzyme.

关 键 词：辣根过氧化物酶 金纳米粒子 纳米生物偶联物 

分 类 号：TB34[一般工业技术—材料科学与工程]