油桐生物素羧基载体蛋白编码基因VfBCCP的克隆与表达分析  

Isolation and Expression Characteristics of Biotin Carboxyl Carrier Protein Coding Gene(VfBCCP) from Vernicia fordii

作　　者：崔琴琴[1] 韩小娇[1] 陈益存[1] 占志勇[1] 林丽媛[1] 汪阳东[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,富阳311400

出　　处：《林业科学》2012年第8期155-160,共6页Scientia Silvae Sinicae

基　　金：国家林业局"948"项目(2009-4-23)

摘　　要：油桐（Vernicia fordii）属于大戟科（Euphorbiaceae）油桐属植物,原产于我国,是世界上著名的木本工业油料树种。其种子榨出的桐油是品质最好的天然干性油之一。桐油具有干燥快、抗酸碱、耐冷热、防腐蚀等特性,作为重要的工业用油广泛应用于制造涂料、高级油墨、增塑剂、医药以及化学试剂等行业（黄挺,2001;Brown et al.,2005）。同时油桐作为生物柴油原料树种具有良好的应用前景（钱能志等,2007;Shang et al.,2010;Chen et al.,2010）。在油桐种子生长发育过程中,7月为桐油的缓慢形成期,7月底到9月初为桐油的迅速累积期,而9月初至10月中旬为桐油累积完成期（王汉涛等,1985）。Biotin carboxyl carrier protein （BCCP） plays an important role as a bridge link in the reaction of Acetyl CoA carboxylase. A full-length eDNA sequence of BCCP gene was obtained from kernel eDNA library, of Vernicia fordii, named VfBCCP （ GenBank accession No. JQ736807）. Sequence analysis indicated that VfBCCP was 1 023 bp in full length with a 5＇-untranslated region（5＇-UTR） of 82 bp, a 3＇-UTR of 182 bp, an opening reading frame（ORF） of 759 bp which encoding 252 predicted amino acids. Furthermore, the genome sequence of BCCP was isolated with 2 157 bp in length which including 5 introns （ GenBank accession No. JX171700）. The amino acid sequence alignment and phylogenetic analysis revealed that VfBCCP had the closest phylogenetie relationship with that of Jatropha curcas belonging to Euphorbiaceae and they shared 85.6% homology. The expression profiles of VfBCCP gene were investigated by employing quantitative real-time RT-PCR method. The results showed that the lower transcription levels of VfBCCP was presented at the early stage of α-eleostearic acid biosynthesis in the middle of July, and rapidly increased during the eleostearic acid accumulation in August, then decreased during accumulation completion period at the end of September. The isolation and expression rule investigation could hold the promise of clarifying VfBCCP function in fatty acid biosynthesis.

关 键 词：油桐 生物素羧基载体蛋白(BCCP) 基因克隆 表达分析 

分 类 号：S718.46[农业科学—林学] S794.3