胃实热证大鼠模型舌象诊断的建立及评价  

Establishment and Evaluation of Tongue Diagnosis in Rat Stomach Real Heat Syndrome Model

作　　者：官宗华[1] 宋捷民[1] 张世亮[1] 丁晓越[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053

出　　处：《中国中医急症》2012年第9期1421-1422,共2页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81073067)

摘　　要：目的建立及评价大鼠胃实热证病理模型及舌象诊断方法。方法采用腹腔注射吲哚美辛(溶于5%NaHCO3液中)10 mg/kg;灌饲辣椒、白酒混合液10 mL/kg,每日2次,造模1周。观察大鼠体征变化及舌外观,运用photoshop图片处理软件对大鼠舌象的色差红色、绿色、蓝色成分进行统计分析,并采用酶联免疫法检测大鼠血清中白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、白细胞介素-10(IL-10)、内皮素-1(ET-1)、环磷酸腺苷(cAMP)、前列腺素E2(PGE2)的含量。结果 1周后可见胃实热组大鼠活动量减少,体质量减少,食量减少,便质黑干硬等症状;舌质红、暗,舌象红色、绿色、蓝色成分数值明显降低,特别是红色成分数值降低更加显著;血清中IL-6、IL-8、IL-10、ET-1含量明显增加,cAMP、PGE2的含量减少。结论采用热因素结合腹腔注射吲哚美辛建立大鼠胃实热证病理模型是可行的,胃实热证病理模型的舌象诊断是有意义的。Objective： To establish and evaluate the tongue diagnosis of rat stomach real heat syndrome model. Methods： The model was established by injection of indometacin （soluble in 5% NaHCO3 liquid, 10 mg/kg） and given a garage of chili liquor mixture （10 mL/kg） with twice per day. After 1 week,the rat signs and tongue ap- pearance were observed and the color difference including red , green and blue were analysed by photoshop image processing software. Some serum indices were detected by the ELISA method such as IL-6,IL-8,IL-10,ET-1, cAMP and PGE2. Results： A week later, the model rats appeared that their movements were reduced and weights were decreased. Meanwhile, their appetites were reduceding and dejecate was black and dry. Their tongues were more red and dark. The number of red, green and blue weres significantly lowered. Especially, the^red was more obviously decreased. Contents including IL-6,IL-8,IL-10 and ET-1 were obviously increased. Levels such as cAMP and PGE2 were significantly lowered. Conclusion： The method of thermal factors combined with injecting indomethacin can establish the rat stomach real heat syndrome model.

关 键 词：胃实热证 大鼠模型 舌象诊断 

分 类 号：R285.5[医药卫生—中药学]