茶扦插苗根腐性苗枯病菌茄镰孢霉专化型研究及相关问题的商榷  

Research on Forma Specialis of Fusarium solani Causing Root Rot of Tea Cutting Seedling and Discussion of Relevant Issues

作　　者：赖传雅[1] 赖传碧 曾凡凯[1] 韦少敏[1] 李复新[1] 周国辉[1] 

机构地区：[1]广西大学农学院,广西南宁530005 [2]桂林茶叶科学研究所,广西桂林541004

出　　处：《云南农业大学学报（自然科学版）》2012年第5期665-669,共5页Journal of Yunnan Agricultural University:Natural Science

摘　　要：茶扦插苗根腐性苗枯病是广西一些茶圃的常见病害,严重影响茶苗出圃。明确该病病原菌分类地位可以为病害防治提供理论依据。为此,作者从桂北茶扦插苗圃中随机采得18株病株标本,分离出镰孢霉纯菌株16株,单孢菌纯系培养18株(GF01～GF18号)。参照BOOTH镰刀菌分类鉴定标准,对代表性单孢菌株GF08号的培养菌进行了形态鉴定;以蘸根法和土壤伤根法测试其致病性,同时在12科22种植物上进行接种试验。结果表明,该菌与茄镰孢霉[Fusarium solani(Mart.)Sacc.]形态特征一致,经柯赫氏法则验证确定其为茶扦插苗根腐性苗枯病的病原菌。该菌除侵染山茶科的福云六号大叶种和白毛茶外,对其余11科20种植物均不致病。因此鉴定该菌为茄镰孢霉一新专化型:Fusarium solani(Mart.)Sacc.f.sp.theae Chuan-Ya Lai etChuan-Bi Lai。Root rot of tea cutting seedling is a common and serious disease occurred in some tea nutseries in Guangxi, China. Determination of taxonomy status of the pathogen can provide theoretic basis for the disease control. So 18 diseased samples were collected from the tea nurseries in north Guilin ofGuangxi, and pure cultures of 16 Fusarium isolates and monospore cultures of 18 strains （GF01 GF18） were obtained. Typical strain GF08 was identified based on BOOTH＇s species concept for Fusarium and tested for pathogenicity by dipping seedling roots and micro injuring roots methods. Twentytwo plant species of 12 families were inoculated with strain GF08. The results showed that the morpho-logical characters of strain GF08 was consistent with Fusarium solani （ Mart. ） Sacc and its pathogenicity was confirmed by Koch＇s postulates. Except for Camellia sinensis and C. sinensis （Theaceae） , thepathogen did not infect other 20 plant species of 11 families. Therefore, the pathogen of root rot of tea cutting seedling was identified as a new forma specialis that was Fusarium solani （Mart.）Sacc. f. sp. theae Chuan-Ya Lai et Chuan-Bi Lai.

关 键 词：茶扦插苗根腐性苗枯病 茄镰孢霉 茄镰孢霉茶专化型 

分 类 号：S435.711[农业科学—农业昆虫与害虫防治]