3种酶液和H_2O_2处理对水曲体胚发生的影响  

Effects of Treatments with Three Types of Oxidase and H_2O_2 Solutions on Somatic Embryogenesis of Fraxinus mandshurica Rupr.(Oleaceae)

作　　者：孙倩[1,2] 杨玲[1,3] 沈海龙[1,3] 李玉花[1,2] 

机构地区：[1]东北林业大学林木遗传育种国家重点实验室,黑龙江哈尔滨150040 [2]东北林业大学生命学院,黑龙江哈尔滨150040 [3]东北林业大学林学院,黑龙江哈尔滨150040

出　　处：《安徽农业科学》2012年第28期13694-13697,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(30872008);中央高校基本科研业务费专项资金(DL10BA04)

摘　　要：[目的]研究3种外源氧化酶液和H2O2处理对水曲柳体胚发生的影响。[方法]利用不同浓度多酚氧化酶(polyphenol oxidase,PPO)、葡萄糖氧化酶(glucose oxidase,GOD)、超氧化物歧化酶(superoxide dismutase,SOD)溶液和过氧化氢(Hydrogen peroxide,H2O2)处理未成熟水曲柳合子胚子叶,探索不同处理对水曲柳体胚发生中子叶外植体生长、褐化以及体胚发生的影响,通过对比分析不同处理在水曲柳体胚发生中的作用,揭示水曲柳体胚发生中外植体褐化与体胚发生的关系。[结果]H2O2处理使水曲柳外植体褐化提前发生,且抑制了外植体的生长和体胚发生;不同浓度PPO处理促进外植体生长和褐化,并提高体胚发生率;GOD和SOD处理均提高外植体的褐化率;经过酶处理的外植体,当体胚发生提前时,各体胚发生率都很低,而当处理30 d的体胚诱导率与处理60 d的体胚诱导率相差较大时,其体胚发生率较高。[结论]该研究结果为提高水曲柳体胚诱导率、改善水曲柳体胚发生状况和优化水曲柳体胚发生系统奠定了基础。[Objective] This study aimed to explore the effects of treatments with three types of exogenous oxidase solutions and H2O2 solution on the somatic embryogenesis of Fraxinus mandshurica Rupr.（Oleaceae）.[Method] The immature zygotic cotyledons were treated with PPO（polyphenol oxidase） solution,GOD（glucose oxidase） solution,SOD（superoxide dismutasesolution） solution and H2O2（hydrogen peroxide） of different concentrations to explore the effects of different treatments on the growth,browning and somatic embryogenesis of cotyledon explants in the somatic embryogenesis of Fraxinus mandshurica Rupr.Through comparative analysis of the effects of different treatments in somatic embryogenesis of Fraxinus mandshurica Rupr.,the relationship between explants browning and somatic embryogenesis was uncovered during the somatic embryogenesis of Fraxinus mandshurica Rupr.[Result] H2O2 treatment not only advanced the explants browning,but also inhibited the growth and somatic embryogenesis of explants;PPO treatment of different concentrations promoted the growth and browning of explants,as well as improving the incidence of somatic embryogenesis;both GOD and SOD treatment could raise the explants browning rate;when somatic embryogenesis of explants treated with enzyme solutions advanced,the incidence of somatic embryogenesis was low;however,when the disparity of the incidence of somatic embryogenesis between 30 d and 60 d treatments reached its peak,the incidence of somatic embryogenesis was also high.[Conclusion] The results of this study provide a basis for raising the incidence and improving the status of somatic embryogenesis of Fraxinus mandshurica Rupr.,as well as optimizing the somatic embryogenesis system of Fraxinus mandshurica Rupr.

关 键 词：水曲柳 体胚发生 PPO GOD SOD 过氧化氢 

分 类 号：S792.38[农业科学—林木遗传育种]