江苏杨梅部分品种的ISSR分析  

Analysis on ISSR Markers of Some Cultivars of Waxberry in Jiangsu Province

作　　者：俞文生[1] 郭志海[1,2] 张林[3] 郄红丽[1] 

机构地区：[1]江苏省太湖常绿果树技术推广中心,江苏苏州215107 [2]苏州农业职业技术学院,江苏苏州215008 [3]南京农业大学,江苏南京210095

出　　处：《安徽农业科学》2012年第28期13731-13733,共3页Journal of Anhui Agricultural Sciences

基　　金：国家公益性行业(农业)科研专项(20120389);苏州市科技支撑(SN201008);苏州市特色水果行业科技公共服务平台(SZP201011);江苏省农业科技自主创新资金项目(CX(12)2011);项目省科技支撑计划农业部分项目(BE2012361);苏州农业职业技术学院自然科学(NS1101)

摘　　要：[目的]研究江苏地区杨梅(Myrica rubra Bieb.et Zucc)的亲缘关系。[方法]用ISSR-PCR方法对18个江苏地区杨梅品种(系)进行分析,并用UPGMA法构建分子系统树。[结果]筛选出的9个引物共扩增出70个DNA片段,其中多态性片段43个,占扩增总片段的61.4%;所分析的18个杨梅品种(系)在遗传距离0.14处分为6组,说明江苏杨梅在基因交流的基础上,有局部隔离。[结论]为杨梅种质资源的保护、利用及新品种的培育提供了科学依据。[ Objective ] This study aimed to investigate the genetic relationship among waxberry （Myrica rubra Bieb. et Zucc ） cultivars in Jiangsu Province. [ Method ] Eighteen waxberry cultivars （lines） in Jiangsu Province were analyzed using ISSR-PCR method, and molecular phylogenetic tree was constructed using UPGMA method. [ Result] A total of 70 DNA fragments were amplified with nine screened primers, including 43 polymorphic fragments, accounting for 61.4% of the total number of amplified fragments; 18 waxberry cultivars （lines） were clustered into 6 categories with the genetic distance of 0.14, indicating that there is partial isolation on the basis of gene exchange between waxben＇y cultivars （lines） in Jiangsu Province. [ Conclusion] This study provided scientific basis for the protection and utilization of waxberry germplasm resources and cultivation of new varieties.

关 键 词：杨梅 ISSR 亲缘关系 

分 类 号：S667.6[农业科学—果树学]