低能离子注入诱变选育盐霉素高产菌株  

Breeding of High-Yield Salinomycin-Producing Streptomyces albus Strain by Low Energy N^+ Ion Beam Irradiation

作　　者：武利勤[1] 苗凤香 顾海科[1] 尚宏忠[1] 

机构地区：[1]北京市辐射中心,北京100875 [2]鲁抗菏泽舍里乐药业有限公司,山东菏泽274016

出　　处：《安徽农业科学》2012年第28期13734-13735,13764,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究N+离子注入对白色链霉菌的诱变效应,同时筛选高产盐霉素的变异菌株。[方法]利用不同剂量的氮离子对白色链霉菌S-11-04菌株进行诱变处理,研究低能氮离子注入对其存活率、菌落形状及产盐霉素能力的影响。[结果]低能氮离子注入对白色链霉菌的诱变效应显著,试验得到了13株盐霉素产量较高的突变菌株,其中N3-6菌株经连续传代4次,遗传稳定性较好,其摇瓶发酵水平较对照提高了41%,发酵生产后平均发酵水平提高了20.5%。[结论]离子注入诱变是获得高产盐霉素突变菌株的有效方法。[ Objective ] This study aimed to explore the mutagenesis effects of N ＋ ion beam irradiation on Streptomyces albus and obtain high- yield salinomycin-producing strain. [ Method ] Streptomyces albus strain S-114）4 was mutated with different doses of N ＋ implantation. The effects of low energy N ＋ implantation on the survival rate, colony shape and salinomycin-producing ability were investigated. [ Resultl The re- suits showed that low energy N ~ implantation can efficiently improve the positive mutation rate of Streptomyces albus; 13 mutant strains with high yield of salinomycin were isolated ; mutant strain N3-6 has good inheritance and fermentation stability with four continuous generations, and the titres of salinomycin were increased by 41% in the shake-flask culture and 20.5% in mass production compared with the control. [ Conclusion] N^＋ ion beam irradiation is an effective method to obtain high-yield salinomycin-producing Streptomyces albus strain.

关 键 词：白色链霉菌 氮离子注入 盐霉素 诱变育种 

分 类 号：S188[农业科学—农业基础科学]