中华芦荟多糖的提取·精制和含量测定研究  

Extraction,Purification and Content Determination of Polysaccharides in Aloe vera L. var chinensis

作　　者：丁晨[1] 郭彬歆[1] 徐琛[1] 

机构地区：[1]南京大学生命科学学院南京大学生物医药国家重点实验室,江苏南京210093

出　　处：《安徽农业科学》2012年第28期14008-14011,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(30600117)

摘　　要：[目的]优化中华芦荟多糖的提取工艺,比较几种精制方法,并建立多糖含量的测定方法。[方法]以多糖得率和含量为评价指标,采用正交试验法对多糖提取工艺进行研究,并比较透析法、AB-8型大孔树脂吸附法和活性炭吸附法3种多糖精制方法;采用芦荟多糖标准品测得芦荟多糖对葡萄糖的换算因子后,用苯酚-硫酸法测定多糖含量。[结果]中华芦荟多糖的最佳提取工艺为料液比1∶10g/ml,提取2次,提取时间3 h;不同精制方法得到的多糖含量:透析法>AB-8型大孔树脂吸附法>活性炭吸附法,多糖收率:活性炭吸附法>透析法>AB-8型大孔树脂吸附法;苯酚-硫酸法测定芦荟多糖对葡萄糖的换算因子为1.32,平均回收率为98.4%,相对标准偏差为2.36%。[结论]优选工艺经济、简单、稳定、可行,透析法精制芦荟多糖简单快速、成本低、多糖含量高,苯酚-硫酸法简便、准确、重现性好。[ Objective ] To optimize the extraction technology of polysaccharides from Aloe vera L. var chinensis, compare several purification methods and establish a method for determining the content of polysaccharides. [ Method] With the yield and content of polysaccharides as the evaluation criteria, the extraction process of polysaccharides was optimized by orthogonal experiment. Three polysaccharides purification methods, including dialysis, macroporous resin AB-8 absorption and activated carbon absorption, were compared. The polysaccharides content was detected eolorimetrically by the phenol-sulfuric acid method after the conversion coefficient of Aloe vera L. var chinensis polysaccharides to glu- cose was obtained. [ Result] The optimal process conditions for extraction were obtained by orthogonal experiment and the optimal parameters were as follows： the solid-liquid ratio of 1：10 g/ml, twice of extraction, and 3 h of extraction time. The contents of polysaccharide purified by the three methods were shown as dialysis 〉 macroporous resin AB-8 〉 activated carbon ; while the yield of polysaceharides was shown as acti- vated carbon 〉 dialysis 〉 macroporous resin AB-8 ; the conversion coefficient of polysaecharide to glucose was determined to be 1.32, the average recovery was 98.4% with a relative standard deviation of 2.36%. [Conclusion] The optimized extraction process is economical, simple, stable and practical, the dialysis method is simple, rapid and cheap to be used for purifying the polysaecharides. The phenol-sulfuric acid method is simple and accurate with good reproducibility.

关 键 词：中华芦荟多糖 提取与精制 工艺比较 含量测定 

分 类 号：S567.239[农业科学—中草药栽培]