梯度离心在体外处理精子结合抗体中的应用  

Application of Gradient Centrifugation In Vitro Treatment of Sperm-binding Antibody

作　　者：刘莉[1] 岳宗相[1] 赖宁[1] 何维[2] 王世衡[2] 张倡惠[1] 

机构地区：[1]成都中医药大学基础医学院微生物免疫教研室,611137 [2]成都中医药大学第二附属医院

出　　处：《国际生殖健康／计划生育杂志》2012年第5期397-398,403,共3页Journal of International Reproductive Health/Family Planning

摘　　要：目的:比较单纯PureSperm方法和PureSperm联合上游法处理精子结合抗体阳性精子的效果。方法:采用单纯梯度PureSperm及PureSperm联合上游法处理精子结合抗体阳性的精子,比较2种处理方法处理后精子结合抗体消退情况。结果:2种方法处理后,精子结合抗体滴度较处理前降低(P<0.01);梯度PureSperm联合上游法处理后,精子结合抗体滴度的下降较单纯PureSperm方法更显著(P<0.05)。结论:采用梯度PureSperm离心或梯度PureSperm离心联合上游法处理精液,可以提高精子质量,再行宫腔内人工授精可能提高免疫性不育的治疗效果。Objective：To compare the PureSperm technique alone and the PureSperm combined with swim-up method in processing the sperm-binding antibody positive sperm.Methods：The PureSperm technique alone and the PureSperm combined with swim-up method were used to process in vitro the sperm-binding antibody positive sperm from patients with male sterility.Results：The titer of sperm-binding antibodies was significantly declined after treatment by both the PureSperm technique（P〈0.01） and the PureSperm combined with swim-up method（P〈0.01）.The titer of sperm-binding antibodies of the PureSperm alone group was significantly lower than that of the PureSperm combination group（P〈0.05）.Conclusions：The sperm quality can be improved by the PureSperm technique alone or combined with swim-up method in those patients with the positive sperm-binding antibodies.The treatment outcome of IUI for patients with immune infertility can be improved when used the sperm samples treated with the PureSperm technique.

关 键 词：不育 男(雄)性 精子 抗体 离心法 梯密度 上游法 

分 类 号：R698.2[医药卫生—泌尿科学]