马鞭草中熊果酸的提取与鉴定  

Extraction and identification of ursolic acid from Verbena

作　　者：李开泉[1,2] 熊曼萍[2] 

机构地区：[1]江西省天然药物活性成分研究重点实验室 [2]宜春学院化学与生物工程学院,江西宜春336000

出　　处：《中国生化药物杂志》2012年第5期606-608,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家"863"计划项目"中药Ⅰ类新药乌索酸及其制剂的研究开发"[2002AA2Z3217]

摘　　要：目的研究马鞭草中熊果酸的制备技术,并建立其含量测定的方法。方法采用"醇提凝析法"提取分离熊果酸,用HPLC法建立其含量测定的方法。结果样品纯度达99.8%,经理化常数测定和光谱学鉴定,确证样品为熊果酸。HPLC法测定含量时,熊果酸在4.6～23.0μg范围内线性关系良好(r=0.999 9),平均回收率为99.82%(RSD为0.75%)。结论本制备技术先进合理,实用可行,有利于工业化生产。HPLC法测定熊果酸含量的方法准确可靠。Purpose To study the extraction technology of ursolie acid from Verbena and establish the method of determining its content. Methods The method of ethanol extraction and agglutination separation was adopted from extracting ursolic acid. The content of ursolic acid in sample is determined by HPLC. Results The purification of ursolic acid is 99.8%. The physieochemieal constants and spectroscopy con- firmed the product of ursolic acid. When determining its content by HPLC, a good linearity can be ob- tained in the range of 4.6-23.0 μg （ r = 0. 999 9） for ursolic acid . The average recovery of ursolic acid is 99.82% with RSD of 0.75%. Conclusion This preparation teehuique is advanced, rational, practical, feasible, and applicable to industrial production. The determination method is accurate and reliable.

关 键 词：马鞭草 熊果酸 提取 结构鉴定 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]