冠心病患者血浆脂联素水平及其基因多态性与小而致密的低密度脂蛋白胆固醇的关系  

Association between plasma adiponectin and small, dense low density lipoprotein in patients withcoronary artery, disease

作　　者：陈刚[1] 秦勤[1] 李杨[1] 任蓓[1] 路雅茹[1] 寇璐[1] 杨宁[1] 冯津萍[1] 赵炳让[1] 

机构地区：[1]天津市胸科医院心内科,300051

出　　处：《中华心血管病杂志》2012年第9期752-756,共5页Chinese Journal of Cardiology

基　　金：天津市卫生局科学基金（2006KRl1）

摘　　要：目的观察冠心病患者血浆脂联素水平及其基因多态性与小而致密的低密度脂蛋白胆固醇（sLDL-C）的关系，进一步探讨其在冠心病发病中的作用。方法将所有受试者分为两组，（1）对照组：250例，男性147例，女性103例，年龄39-81（60．26±7．52）岁。（2）冠心病组：267例，男性153例，女性114例，年龄37-77（60．79±9．63）岁。血浆脂联素水平采用竞争酶联免疫方法（ELISA）测定；血浆sLDL-C和氧化低密度脂蛋白（OX-LDL）采用ELISA的方法测定；采用多聚酶链反应限制性内切酶片段长度多态性（PCR。RFLP）分析脂联素SNPrsl501299和rs266729基因多态性。结果（1）冠心病组血浆脂联素水平明显低于对照组[（306．17±74．52）mg／L比（321．78±86．28）mg／L]，而sLDL-C和OX-LDL的血浆水平则明显高于对照组[（276．30±45．55）ng／L比（249．00±32．02）ng／L和（545．06±115．46）μg/L比（497．74±106．09）μg/L，P〈0．05]。logistic回归分析结果显示脂联素、sLDL．C和年龄与冠心病密切相关。（2）脂联素的血浆水平与sLDL-C呈显著负相关，sLDL-C和OX-LDL呈正相关。（3）脂联素SNPrsl501299和rs266729的基因型和等位基因频率分布在对照组和冠心病组之间无明显差异。（4）脂联素2个位点的基因型和血浆脂联素、sLDL-C和OX-LDL水平在两组间无明显差异。结论脂联素和sLDL-C呈负相关，低脂联素水平和高sLDL-C水平是冠心病的独立危险因素，脂联素SNPrsl501299和rs266729基因多态性与冠心病的发病无明显相关。Objective To observe the association between adiponectin and small dense low-density lipoprotein （sLDL-c） in coronary artery disease （CAD） patients. Furthermore, we sought to determine the association between single nucleotide polymorphisms （SNP） rs1501299 （＋ 276G/T）, rs266729 （-11365C/G） and the incidence of CAD. Methods Consecutive subjects with ehest discomfort were examined by coronary angiography and divided into non-CAD E n = 250, 147 male, mean age （ 60. 26 ± 7. 52） yearsl and CAD En =267, 153 male, mean age （60. 79 ＋9.63） years] groups. Blood samples were collected from all participants following an overnight fasting for at least 12 hours. Plasma adiponectin levels were measured by competitive enzyme-linked immunosorbent assay （ ELISA ）. The serum levels of sLDL-C and oxidized low-density lipoprotein （ox-LDL） were determined by ELISA. Genotypes in rs1501299 and rs266729 of the adiponectin were determined by polymerase chain reaction （ PCR ） . Results 1. The adiponectin levels were significantly lower [ （ 306. 17 ± 74. 52 ） mg/L vs. （ 321.78 ± 86. 28 ） mg/L ] , whereas sLDL-C and ox-LDL levels were significantly higher I （ 276. 30 ± 45.55 ） ng/L vs. （ 249. O0 ± 32.02）ng/L and （545.06 ± 115.46） Ixg,/L vs. （497.74 ± 106. 09） txg/L,P 〈0. 051 in CAD group than non-CAD group. 2. Adiponectin level was negatively associated with sLDL-C, whereas sLDL-C positively correlated with ox-LDL in all subjects. 3. Genotype distribution and allele frequencies of rs1501299 and rs266729 were similar between CAD and non-CAD subjects and not related to the serum levels of adiponeetin, sLDL-C and ox-LDL.

关 键 词：冠状动脉疾病 脂联素 脂蛋白类 LDL 多态性 单核苷酸 

分 类 号：R541[医药卫生—心血管疾病]