成年斑马鱼OKR行为学分析  

Analysis of adult zebrafish OKR behavior

作　　者：黄玉斌[1] 邹苏琪[1] 殷梧[1] 王昆[1] 王晗[2] 胡兵[1] 

机构地区：[1]中国科学技术大学生命科学学院脑功能与脑疾病重点实验室,合肥230027 [2]苏州大学医学院生物钟研究中心,苏州215123

出　　处：《遗传》2012年第9期1193-1201,共9页Hereditas（Beijing)

基　　金：国家重点基础研究发展计划项目(973项目)(编号:2011CB504402;2012CB947600);国家自然科学基金项目(编号:31070950)资助

摘　　要：作为视功能检测和与视觉有关突变体筛选的方法,眼动(Optokinetic response,OKR)和视动(Optomoterresponse,OMR)行为学是简单有效的视功能检测手段,广泛用于幼年斑马鱼研究中,而成年斑马鱼OKR的分析方法却很少有报道。文章介绍了成年斑马鱼眼动反应诱导方式,以及使用模板匹配(Pattern match)的方法程序跟踪眼部运动,实现了成年斑马鱼OKR的定量分析。使用该方法,检测到斑马鱼双眼视觉区对OKR行为的产生具有一定的贡献作用,并且成年斑马鱼单眼对运动光栅表现出一定的方向敏感性。同样的方法也可适用于幼年斑马鱼的OKR行为学分析。利用此方法初步检测到了钟基因period1b突变体幼鱼的OKR异常。As a means of visual function testing and visual related mutants screening, the optokinetic response （OKR） and the optomotor response （OMR） behaviour tests are simple and effective tools for visual functional testing, which have been widely used in studying zebrafish larvae. However, adult zebrafish OKR analysis method is rarely reported. In this study, the methods of inducing adult zebrafish OKR behaviour, as well as tracking the movement of eyes using Pattern Match approaches, are presented. The quantitative measurement of the adult zebrafish OKR behaviour was successfully established. Using these methods, the binocular vision area was found to make a certain contribution to OKR behaviour. Moreover, the monocular vision of adult zebrafish showed a certain degree of directional sensitivity to moving gratings. Such approaches can also be applied to the zebrafish larvae OKR. The abnormal OKR behaviour phenomenon ofperiodlb mutant larvae fish was detected.

关 键 词：成年斑马鱼 OKR 视动反应 行为学 幼鱼 模板匹配 

分 类 号：Q436[生物学—生理学]