黄瓜ζ-胡萝卜素脱氢酶基因克隆及表达分析  

Molecular Cloning and Expression Analysis of ζ-Carotene Desaturase Gene from Cucumis sativus L.

作　　者：王柬人[1] 李锡香[1] 王海平[1] 邱杨[1] 宋江萍[1] 张晓辉[1] 沈镝[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,农业部蔬菜作物基因资源与种质创制北京科学观测实验站,北京100081

出　　处：《分子植物育种》2012年第5期520-527,共8页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31071797);农业部园艺作物生物学与种质创制重点实验室项目共同资助

摘　　要：以西双版纳黄瓜为试材，从黄瓜果肉中克隆ζ-胡萝卜素脱氢酶（ZDS）基因的cDNA序列，命名为CsZds。获得目的片段长度为1805bp，该序列具有完整的开放阅读框，位于31～1761bp，编码576个氨基酸。序列比对分析结果显示，该蛋白在氨基端变化十分明显，中部较为保守，存在一个特征序列。系统进化分析表明，推测的CsZDS蛋白与南瓜中ZDS蛋白同源性最高，达到91．84％。利用实时荧光定量PCR技术分析结果显示，随着果实成熟期的延长，西双版纳黄瓜CsZds基因的表达量呈明显上升的趋势，在转色期达到最大。在果实发育过程中，西双版纳黄瓜CsZds基因的表达量高于普通黄瓜。A 1 805 bp target cDNA of ζ-Carotene desaturase （ZDS） was amplified and cloned from the fruit flesh of Cucumis sativus L. var. xishuangbannanesis, and it was named as CsZds. The sequence had a full open reading frame located from 31 bp to 1 761 bp encoding for 576 amino acids. Sequence comparison analysis showed that ZDS protein presented clear variation at N-terminus, but it was highly conserved in the mid-section with one characteristic sequence. Phylogenetic analysis revealed that deuced CsZDS protein was closest to ZDS protein in pumpkin, with 91.84% similarity. Real-time PCR analysis indicated that with the expression of fruit development period, the expression level of CsZds gene in xishuangbannanesis showed significant increase, and reached the highest in color change stage. During the development of fruit maturation, the expression level of CsZds gene in Cucumis sativus L. var. xishuangbannanesis was higher than that in common cucumber （C. sativus L.）.

关 键 词：西双版纳黄瓜 ζ-胡萝卜素脱氢酶 基因克隆 表达 

分 类 号：S642.2[农业科学—蔬菜学]