农杆菌介导OsWRKY45基因转化油菜的研究  

Agrobacterium-mediated Transformation of Brassica napus with OsWRKY45 Gene

作　　者：曲晓言[1] 彭少丹[2] 汪骞[3] 陈升位[1] 孟霖[1] 林良斌[1] 

机构地区：[1]云南农业大学农学与生物技术学院,昆明650201 [2]云南农业大学农科专业实验教学中心,昆明650201 [3]云南省农业科学院园艺研究所,昆明650205

出　　处：《分子植物育种》2012年第5期541-545,共5页Molecular Plant Breeding

基　　金：国家支撑计划(2011BAD35B04);国家863计划(2011AA10A104)共同资助

摘　　要：干旱严重影响油菜的生长,降低油菜籽的产量,造成严重的经济损失。利用农杆菌介导法把具有抗旱作用的OsWRKY45基因转入油菜获得到转基因植株,经PCR检测OsWRKY45基因已整合到油菜基因组DNA。不同品种的不同外植体的转化率不同,花油7号的抗性植株获得率稍高于湘油15,下胚轴的抗性植株获得率是愈伤组织的2倍,花油7号下胚轴的抗性植株获得率高达10.9%。干旱胁迫表明高表达OsWRKY45转基因植株的抗旱性有明显的提高。The growth and yield Of rapeseed are severely affected by drought, which will result in considerable economic loss. A drought-resistant gene OsWRKY45 mediated by Agrobacteria was transformed into Brassica napus Huayou7 and Xiangyoul5. PCR detection verified that the OsWRKY45 was integrated into the rapeseed genome and the：：transgenic plants were obtained. The transformation rates of the different explants from different varieties are different, and the percentage of kanamycin-resistant plants from Huayou7 is slightly higher than that from Xiangyoul5, while that from the hypocotyls is twice as that from the callus. Moreover, the percentage of kanamycin-resistant plants from the hypocotyls of Huayou7 reaches up to 10.9%. Drought-resistant ability of transgenic plants is much higher than that ofnon-transgenic plants.

关 键 词：油菜 OsWRKY45基因 转基因植株 分子检测 

分 类 号：S565.4[农业科学—作物学]