一个与水稻直立穗基因qPE9-1关联的InDel标记的设计与验证  

Development and Application for a Functional Marker for Erect Panicle Gene qPE9-1 of Rice

作　　者：朱金燕[1] 王军[1] 杨杰[1] 范方军[1] 杨金欢[2] 仲维功[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所,江苏省优质水稻工程技术研究中心,国家水稻改良中心南京分中心,南京210014 [2]安徽农业大学生命科学院,合肥230036

出　　处：《分子植物育种》2012年第5期583-588,共6页Molecular Plant Breeding

基　　金：国家与江苏省粮食丰产科技工程(2011BAD16B03/BE2009425);江苏省农业科技自主创新基金(CX[11]4020)共同资助

摘　　要：水稻穗型是重要的农艺性状,也是水稻高产的重要决定因素之一。本研究根据水稻弯曲穗品种与直立穗品种在直立穗基因qpe9-1第5外显子处存在的核苷酸差异设计了1对插入-缺失标记(Insert/Delection),InDel-E5。利用该功能标记对24份水稻品种资源材料及直立穗分离群体F2进行标记基因型分析,研究结果表明,直立穗品种均表现为缺失带型,弯曲穗品种均表现为非缺失带型,F2群体中杂合带型和非缺失带型均为弯曲穗,而缺失带型均为直立穗,说明利用该标记可以快速、准确的鉴定出直立穗品种和单株。Rice plant architecture is an important agronomic trait and a major determinant in high productivity. In this study, one InDel marker, InDel-ES, was designed based on the difference of extron 5 between curved panicle gene qPE9-1 and erect panicle qpe9-1.24 rice varieties and F2 segregated populations were detected for genotype analysis using InDel-E5 marker. The results showed that InDel-E5 was cosegregated with the rice panicle erectness, allowing to identify the homozygous erect panicle genotype, homozygous curved panicle genotype and heterozygous curved panicle genotype individuals. Therefore, InDel-E5 can be used to determined rice panicle genotype rapidly and exactly.

关 键 词：水稻 直立穗 弯曲穗 功能标记 

分 类 号：S511[农业科学—作物学]