缬沙坦对血管损伤小鼠血管重构的影响  

Influence of valsartan on vascular remodeling in mice with vascular injury

作　　者：王筱梅[1] 刘少奎[1] 陈士毅[1] 洪军[1] 穆鑫[1] 王翠荣[1] 赵越超[1] 

机构地区：[1]大连大学附属中山医院心内科,116001

出　　处：《中国循证心血管医学杂志》2012年第4期363-365,共3页Chinese Journal of Evidence-Based Cardiovascular Medicine

摘　　要：目的观察缬沙坦对血管损伤小鼠血管壁内膜、中膜的影响。方法 8周龄雄性C57BL/6野生型小鼠80只,随机分为手术组(n=20)、治疗组(n=20)、假手术组(n=20)和对照组(n=20)。手术组:行聚乙烯套管包裹股动脉手术,术后开始蒸馏水灌胃治疗(1mL/次,qd);治疗组:行聚乙烯套管包裹股动脉手术,术后开始缬沙坦灌胃治疗(10mg/kg,qd);假手术组:游离出股动脉不套管,术后不灌胃;对照组:不手术,不灌胃。于手术后第14天处死小鼠,取各组损伤套管处股动脉(对照组取相应部位股动脉);采用苏木素-伊红(HE)染色,光镜下观察血管形态学变化,NIH分析软件测量新生血管内膜和中膜的面积。结果各组中膜均无增厚表现,与术前相比差异无统计学意义(P>0.05)。假手术组及对照组无新生内膜增厚,与实验前相比差异无统计学意义(P>0.05);手术组与治疗组内膜均增厚、面积增大,其中手术组内膜面积增厚程度最大,但与治疗组相比差异无统计学意义(P>0.05)。手术组与假手术组及对照组相比,内膜面积比较差异统计学意义(P<0.05)。结论短期应用缬沙坦能一定程度上抑制损伤后血管内膜增厚、抑制血管重构。Objective To observe the influences of valsartan on intima and tunica media in mice with vascular injury. Methods Male C57BL/6 wild mice （8 weeks old, n-80） were randomly divided into operation group, treatment group, sham-operation group and control group （each n=20）. The operation group was treated intragastrieally with distilled water （1 mL/time, qd） after femoral artery operation with polyethylene tube around, and treatment group, intragastrically valsartan （10 mg/kg, qd）. In sham-operation group femoral artery was liberated without polyethylene tube around and intragastrical medicinal. The control group was not given operation and intragastrical medicinal. On the 14th day, all mice were sacrificed and injured femoral artery was taken from tube place （from corresponding place in control group）. The changes of vascular morphology were observed with light microscope after HE staining. The areas of intima and tunica media of new vessels were measured by using NIH analysis software. Results The tuniea media was not thickened in all groups and the difference was not statistically significant compared with that before treatment （P〉0.05）. The intima was not thickened in sham- operation group and control group and the difference was not statistically significant compared with that before treatment （P〉0.05）. The intima was thickened and intima area was enlarged in operation group and treatment group, and the thickening was the most significant in operation group but the difference was not statistically significant compared with treatment group （P〉0.05）. The difference in intima area compared between operation group or sham-operation group and control group was statistically significant （P〈0.05）. Conclusion The short- term administration of valsartan can inhibit the intima thickness of injured vessels and vascular remodeling to some extent

关 键 词：血管重构 缬沙坦 血管损伤 

分 类 号：R972.4[医药卫生—药品]