肿瘤坏死因子-α和血浆纤溶酶原激活抑制物-1与动脉粥硬化相关性研究  

The Relationships Between Tumor Necrosis Factor-α and Plasminogen Activator Inhibitor-1 of adipose tissue with Atherosclerosis

作　　者：林红[1] 

机构地区：[1]新疆医科大学附属中医医院心内二科,新疆乌鲁木齐830000

出　　处：《中国实验诊断学》2012年第9期1622-1625,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨脂肪组织中肿瘤坏死因子-α(TNF-α)、血浆纤溶酶原激活抑制物-1(PAI-1)mRNA转录量和蛋白表达量与动脉粥样硬化的相关性,从而揭示脂肪组织中TNF-α、PAI-1用于动脉粥样硬化分型的临床价值。方法选取60例动脉粥样硬化患者,按照病灶类型分为A组(I型病灶)、B组(Ⅱ型病灶)、C组(Ⅲ型病灶)、D组(Ⅳ型病灶)、E组(V型病灶)和F组(Ⅵ型病灶)。使用RT-PCR检测6组患者脂肪组织中TNF-α、PAI-1mRNA转录量(以β-actin为内参),使用Western Blot检测6组脂肪组织中TNF-α、PAI-1蛋白表达量(以β-actin为内参)。分析TNF-α、PAI-1mRNA转录量和蛋白表达量之间的相关性。结果随着动脉粥样硬化病灶严重程度的增加,TNF-α、PAI-1mRNA转录和蛋白表达水平均显著增加(P均<0.05),且TNF-α、PAI-1mRNA转录和蛋白表达水平具有显著正相关性(r=0.9488,P<0.01;r=0.9659,P<0.01)。结论脂肪组织TNF-α、PAI-1mRNA转录水平和蛋白表达水平与动脉粥样硬化病灶分型具有正相关性,且TNF-α和PAI-1呈正相关性。脂肪组织TNF-α、PAI-1检测对动脉粥样硬化病灶分型具有诊断价值。。Objective To mesure TNF-α and PAI-1 mRNA transcript levels and protein expression in adipose tissue,which revealed the correlation between TNF-α and PAI-1 with atherosclerosis.Methods 60 patients with atherosclerosis were divided into group A（type I lesions）,group B（type II lesions） and Group C（III lesions）,group（IV lesions）,E group（V-shaped lesions） and F（VI lesions） according to lesion type.RT-PCR was used to detect TNF-α and PAI-1 mRNA transcript levels（β-actin was the internal reference）,and Western Blot was used to measure TNF-α and PAI-1 protein expression levels in adipose tissue（β-actin was the internal reference）.Analysis of the correlation between the TNF-α and PAI-1 mRNA transcript levels and protein expression levels were performed.Results As the severity of lesions were increasing,TNF-α and of PAI-1 mRNA transcription and protein expression levels increased significantly（P 0.05）,and TNF-α and PAI-1 mRNA transcription and protein expression levels have significantly positive correlation（r = 0.9488,P〈0.01;r = 0.9659,P 0.01）.Conclusion Adipose tissue TNF-α and PAI-1 mRNA transcription and protein expression levels correlated with atherosclerosis sclerosis lesion type positively,with TNF-α and PAI-1 positively correlated.Adipose tissue TNF-α and PAI-1 detection may have the diagnostic value of atherosclerosis sclerosis lesion sub-type.

关 键 词：肿瘤坏死因子-Α 血浆纤溶酶原激活抑制物-1 动脉粥样硬化 总胆固醇 

分 类 号：R543.5[医药卫生—心血管疾病]