萝卜胞质雄性不育系小孢子母细胞发育的组织学观察  

Histological Observation of Microsporogenesis in Cytoplasm Male Sterile(CMS) Line of Radish(Raphanus sativus L.)

作　　者：张一卉[1] 谭婷婷[1,2] 刘立锋[1] 林超[1,2] 李化银[1] 李利斌[1] 王淑芬[1] 程斐[2] 高建伟[1] 

机构地区：[1]山东省农业科学院蔬菜研究所/山东省设施蔬菜生物学重点实验室/国家蔬菜改良中心山东分中心,山东济南250100 [2]青岛农业大学园林园艺学院,山东青岛266109

出　　处：《山东农业科学》2012年第9期17-21,共5页Shandong Agricultural Sciences

基　　金：山东省现代农业产业技术体系;山东省良种工程"以生物技术为主要手段的蔬菜种质资源创新利用研究"项目资助

摘　　要：利用石蜡切片的方法对萝卜雄性不育系RS-CMS2的小孢子母细胞发育过程进行了组织学观察。结果表明:萝卜雄性不育株在四分体时期开始出现败育现象;绒毡层细胞膨大,出现大的液泡;随后绒毡层细胞破裂,侵入药室,细胞彼此之间难以分清界限;小孢子粘连在一起,胞质进一步收缩,并开始出现降解。该研究为进一步揭示萝卜细胞质雄性不育的作用机制、提高育种效率有重要的参考价值。The paraffin method was used for histological observation of microsporogenesis of the male sterile line RS - CMS2. The male sterile line began to abort when mierospores at tetrad stage. The tapetal cells enlarged abnormally at meiotic phase and presented many large vacuoles. Then with the degeneration of the ta- petal cells, mierospores conglutinated each other and fused to form a big conglomeration. Finally, the pollen grains aborted completely. This result would be helpful to understand the mechanism of cytoplasm male sterile （CMS） and to increase the breeding efficiency in the future.

关 键 词：萝卜 雄性不育 小孢子母细胞 组织学观察 

分 类 号：S631.101[农业科学—蔬菜学]