CE-LIF在氨基甲酸酯类农药研究中的应用  

Study on carbamate pesticides by capillary electrophoresis with laser-induced fluorescence detection

作　　者：杨玉林 赵晓寅[2] 赵文杰[2] 周天舒[2] 

机构地区：[1]通标标准技术服务(上海)有限公司,上海200233 [2]华东师范大学环境科学系,上海200062

出　　处：《华东师范大学学报（自然科学版）》2012年第5期54-61,共8页Journal of East China Normal University(Natural Science)

基　　金：上海市自然科学基金(11ZR1410700)

摘　　要：用毛细管电泳激光诱导荧光检测法(CE-LIF),对灭除威(XMC)、混杀威(2,3,5-trimethacarb)和灭虫威(mercaptodimethur)等3种氨基甲酸酯类农药进行了研究.在碱性条件下,氨基甲酸酯类农药水解产生甲胺并与荧光衍生试剂4-氯-7-硝基-2,1,3-苯并二唑(NBD-Cl)发生衍生化反应,产物经毛细管电泳激光诱导荧光检测法测定.对影响水解、衍生以及毛细电泳分离的因素如缓冲液酸度、浓度和衍生试剂的浓度等进行了详细讨论;在最佳条件下,3种农药的检出限分别可达3.0×10^(-9)、1.0×10^(-9)和3.0×10^(-9)mol/L,并测定了它们的水解速率常数及活化能.该方法不仅可对氨基甲酸酯类农药进行测定,同时也可用于对这类农药的一些理化常数的测定.A sensitive and rapid capillary electrophoresis method with laser-induced fluorescence detection （CE-LIF） was developed for the determination of carbamate pesticides （including （3,5- dimethylphenyl） N-methylcarbamate （XMC）, and mercaptodimethur）. In alkaline solution, car- bamate was hydrolyzed to methylamine, which can be derivatized by 4-chloro-7-nitrobenzo-2-oxa- 1,3-diazole （NBD-C1） and detected by CE-LIF. Several parameters which influence the hydroly- sis, derivatization and electrophoresis separation, such as pH, concentration of buffer solution and concentration of NBD-C1, were investigated. The detection limits of the three carbamates were 3.0 ×10^-9 , 1.0 ×10^-9 and 3.0×10^-9 mol/L, respectively. The proposed method was suc- cessfully employed to determine the rate constants and activation energies for carbamates hydrol- ysis.

关 键 词：毛细管电泳 激光诱导荧光 氨基甲酸酯类农药 水解速率常数 活化能 

分 类 号：Q657[生物学—生物物理学]