肺表面活性物质对肺损伤胎兔肺组织转化生长因子β1表达的影响  

Effect of pulmonary surfactant on the expression of TGF-β1 in fetus lung injury in rabbit model with intrauterine infection

作　　者：吴静[1,2] 刘冬云[3] 刘敬[2] 封志纯[2] 

机构地区：[1]南方医科大学附属何贤纪念医院新生儿科,广东广州511400 [2]北京军区总医院附属八一儿童医院新生儿中心,山东青岛266003 [3]青岛大学医学院附属医院新生儿科,北京100700

出　　处：《广州医学院学报》2012年第3期9-13,共5页Academic Journal of Guangzhou Medical College

基　　金：中国博士后科学基金(20080431405);中国博士后科学基金特别项目(200801041)

摘　　要：目的:探讨羊膜腔内注射肺表面活性物质(PS)对宫内感染致肺损伤胎兔的肺组织转化生长因子β1(TGF-β1)表达的影响。方法:选取健康成年育龄日本大耳白兔19只,成功受孕后随机分为对照组、细菌组、细菌+PS组,建立宫内感染模型。对照组和细菌组按胎龄又分为21、22、23、24、26 d 5个亚组,细菌+PS组分为24、26 d 2个亚组。细菌组宫内注射E.coli菌株,细菌+PS组宫内注射E.coli菌株的同时羊膜腔内注射PS200 mg/kg,对照组宫内注射生理盐水。模型建成后分别在各时相点取胎兔肺组织,免疫组织化学法检测肺组织内TGF-β1表达;RT-PCR检测TGF-β1mRNA表达;Western blotting法检测TGF-β1蛋白表达。结果:对照组TGF-β1蛋白在支气管上皮或血管内皮细胞及其周围的结缔组织有阳性表达,细菌组、细菌+PS组TGF-β1阳性蛋白表达量增加,与对照组比较,差异有统计学意义(P<0.05)。TGF-β1mRNA、蛋白表达在细菌感染后明显上调,3组比较,差异有显著统计学意义(F=631.109、183.980,P=0.000)。结论:宫内感染后胎肺TGF-β1高表达是肺损伤的高危因素。羊膜腔内注射PS可使TGF-β1表达下降,对减轻肺损伤、促进肺发育具有积极作用。Abstract Objective：To investigate the effect of Intra-amniotic administration of pulmonary surfactant on the expression of TGF-B1 in fetus lung injury in rabbit model with intrauterine infection. Methods： Nineteen Japanese albino pregnant rabbits were divided into 3 groups： bacteria group , bacteria ＋ PS group and control group. The rabbit models of fetus lung injury with intrauterine infection were established. The control group and bacteria group were subdivided into 21 d, 22 d, 23 d, 24 d, 26 d fetation group respectively, and the bacteria ＋ PS group were subdivided into 24 d and 26 d fetation group. In the bacteria group, the rabbits were given an intrauterine injection of Escherichia coli; in the bacteria ＋ PS group, the rabbits received an intrauterine administration of Eseheriehia coli and intra-amniotie injection of PS 200 mg/kg; the rabbits of the control group were.jniected with 0.9% sodium chloride. All the rabbits were delivered bv cesarean section and the fetus werataken. Lung tissues of fetus rabbits were excised at each time points. Immunohistochemistry method was used to detect TGF-[31 expressions in lung tissues. The expression of TGF-B1 mRNA in lung tissues were determined by reverse transcription-polymerase chain reaction and TGF-B1 protein by Western blotting. Results：The positive expression of TGF-B1 protein in bronchial epithelium or vascular endothelial cells and connective tissues could be observed in the control group. Compared with the control group, the expression of TGF-B1 in the bacteria group and bacteria ＋ PS group was significantly increased（P 〈0.05）. The expression of TGF-B1 mRNA and protein significantly up-regulated after bacterial infection, and the differences among the three groups were statistically significant （ F = 631. 109, P = 0. 000）. Conclusions： After intrauterine infection, high expression of TGF-B1 in fetal lung tissues is a dangerous factor of lung injury. Intra-amniotic injection of PS can decrease the expression of TGF-B1 ,which might

关 键 词：肺表面活性物质 肺损伤 宫内感染 转化生长因子B1 

分 类 号：R563[医药卫生—呼吸系统]