大鼠自体原位肝移植肺组织TNF-α、IL-1β和IL-6的表达  

TNF-α,IL-1β,and IL-6 Genes Transcription and Expression Level of Lung Tissue in Rats Experienced Orthotopic Autologus Liver Transplantation

作　　者：罗刚健[1] 池信锦[1] 张瑷兰[1] 朱国松[1] 黑子清[1] 

机构地区：[1]中山大学附属第三医院麻醉科,广东广州510630

出　　处：《中山大学学报（医学科学版）》2012年第4期444-448,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(30972858)

摘　　要：【目的】观察大鼠自体原位肝移植围术期肺组织TNF-α、IL-1β和IL-6基因表达和水平的变化。【方法】选取健康SPF级SD大鼠30只,随机分为假手术对照组(S组,n=6)和移植(肝脏再灌注)后4、8、16、24 h组(M4、M8、M16和M24组,每组n=6)。观察肺组织病理损伤,RT-PCR检测肺组织TNF-α、IL-1β和IL-6基因表达,ELISA分析肺组织匀浆TNF-α、IL-1β和IL-6水平。【结果】①病理学检查:假手术组肺组织结构基本正常,移植组表现为肺间质明显出血、充血,中性粒细胞浸润显著增多,以肝脏再灌注后8 h最为明显。②与S组相比,M4、M8、M16、M24组肺含水率显著升高,TNF-α、IL-1β及IL-6的基因表达均显著上调(P<0.05),在肝脏再灌注8h达到峰值。③与S组相比,M4、M8、M16、M24组肺组织匀浆TNF-α、IL-1β及IL-6水平显著升高(P<0.05);TNF-α、IL-1β在肝脏再灌注8h达到峰值,而IL-6则在肝脏再灌注后16 h达到峰值。【结论】大鼠自体原位肝移植围术期肺组织TNF-α、IL-1β和IL-6浓度显著升高,基因表达水平增高,可能与围术期发生急性肺损伤有关。[Objective] To investigate the gene transcription and expression level of TNF-α、IL-1βandIL-6 in lung tissue of rats experienced orthotopic autologus liver transplantation. [ Method] Thirty healthy SPF Sprague-Dawley rats were randomly divided into five groups. According to the different times after liver reperfusion, the rats were divided into sham group （S, n = 6）, 4 h after liver reperfusion group （M4, n = 6）, 8 h after liver reperfusion group （M8, n=6）, 16 h after liver reperfusion group （M16, n=6）, 24 h after liver reperfusion group （M24, n = 6）. Lung tissues of the each animal in different groups were collected and sent to analyze tile morphology change as well as he genes transcription and expression level of TNF-α、IL-1βandIL-6. RT-PCR was adopted to evaluate the TNF-α、IL-1βandIL-6 genes transcription level and ELISA method was performed to determine the TNF-α、IL-1βandIL-6 genes expression level in lung tissue. [ Results ] （ 1 ） Lung tissue histological analysis shown the rats in sham group exhibited the normal structure of the lung tissue and seldom inflammatory cells infiltration, while the rats received orthotopic autologus liver transplantation revealed collapse of the pulmonary structure, severely infiltration of inflammatmy cells into the intra-alveolar and interstitial spaces as well as edematous interstitial space. （2） Comparing with sham group, lung water content and genes transcription level of TNF-α、IL-1βandIL-6 in M4, Ms, Mr6 and M24 groups significantly increased （P 〈 0.05）, while each of them reached the peak value at 8h after liver reperfusion. （3） Comparing with sham group, genes expression level of TNF-α、IL-1βandIL-6 in M4, Ms, MI6 and M24 groups significantly increased （P 〈 0.05）, TNF-α、IL-1βapproached the top value at 8 h after liver reperfusion, while IL-6 reached the peak value at 16 h after liver reperfusion. [Conclusion] The genes transcription and expression level of TNF-α、IL-1βandIL-6 in lung tissue of the r

关 键 词：自体原位肝移植 肺 炎症因子 

分 类 号：R657.3[医药卫生—外科学]