三种抗生素对德国小蠊肠道菌去除效果的研究  

A STUDY OF THE REMOVAL OF INTESTINAL FLORA IN BLATTELLA GERMANICA USING THREE ANTIBIOTICS

作　　者：刘濯[1] 张凡[2] 黄艳红[1] 霍新北[1,2] 

机构地区：[1]山东师范大学生命科学学院,济南250014 [2]山东省疾病预防控制中心,济南250014

出　　处：《山东师范大学学报（自然科学版）》2012年第3期115-117,120,共4页Journal of Shandong Normal University(Natural Science)

基　　金：山东省教育厅科技发展计划项目（J11LC13）;山东省医药卫生科技发展计划资助项目（2011HZ056）.

摘　　要：利用稀释涂布法和PCR法评价了3种抗生素利福平、庆大霉素和青霉素在不同的浓度（50．0、100．0及200．0μg／m1）对德国小蠊肠道菌的去除效果．结果表明：德国小蠊肠道菌射庆大霉索和利福平敏感，对青霉素不敏感．在不影响德国小蠊正常生活的情况下，200．0ixg／ml浓度时，庆大霉索对德国小蠊肠道菌的去除效果最好，利福平次之，青霉素最差．本研究将为“无肠道菌”德国小蠊种群的建立以及虫体与肠道菌之间关系的进一步研究提供便利．Dilution - plate method and PCR method were used to evaluate the removal efficiency of intestinal flora in Blattella germanica using three antibiotics, rifampicin, gentamicin and penicillin in different concentrations （50.0, 100.0 and 200.0 μg/ml）. The results showed that intestinal flora in Blattella germanica were sensitive to gentamiein and rifampicin, not so sensitive to penicillin, At a concentration of 200.0 μg/ml, gentamicin and rifampicin can efficiently remove the intestinal flora without causing the normal life of Blattella germanica and penicillin was not. The study will obtain the intestinal flora free population of Blattella germanica and facilitate the further study in relation between the insect and intestinal bacteria.

关 键 词：德国小蠊 肠道菌 利福平 庆大霉素 青霉素 

分 类 号：Q96[生物学—昆虫学]