脂质体结构稳定性的原子力显微镜观测与软球数学模型分析  

Stability of liposome structure studied by atomic force microscope and a soft sphere model analysis

作　　者：赵琰[1] 孙润广[1] 张小飞[1] 

机构地区：[1]陕西师范大学物理学与信息技术学院,陕西西安710062

出　　处：《陕西师范大学学报（自然科学版）》2012年第5期27-30,共4页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(20772077);教育部高等学校博士点专项科研基金资助项目(200807180001)

摘　　要：利用原子力显微镜和激光粒度分析仪,研究手摇法和超声薄膜法制得脂质体的粒度特性、粘连性与刚性特性.结果表明:手摇法制得脂质体粒径较大、易粘连且刚性较弱.超声薄膜法制得脂质体粒径较小、分散性较好且刚性较强.根据脂质体本身的特性建立了软球数学模型,进行粘连性、刚性的理论分析,并将实验测得的数据带入模型计算,理论计算与实验观测结果一致,表明软球数学模型可为脂质体的结构稳定性提供定量的评价标准.The particle size,adhesion and rigidity of liposome obtained with hand-shaking method and ultrasonic-filming method were studied by atomic force microscope（AFM）.The liposome has a larger particle size with adhesion tendency and weak rigidity compared with that of the liposome obtained by ultrasonic-filming method.According to characteristics of liposome,a soft sphere model was established and the adhesion and rigidity were analyzed theoretically. The results were consistent with that of the experimental observations.This proves that the liposome can be studied as a soft sphere model,and this model provides a quantitative evaluation criterion for the structural stability of the liposome.

关 键 词：原子力显微镜 脂质体 刚性 软球数学模型 

分 类 号：Q502[生物学—生物化学]